Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 172961..173565 Replicon chromosome
Accession NZ_CP020089
Organism Enterobacter cloacae strain PIMB10EC27

Toxin (Protein)


Gene name hicA Uniprot ID A0A421IJD5
Locus tag B2J95_RS00855 Protein ID WP_071843252.1
Coordinates 172961..173146 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7Z1F6W0
Locus tag B2J95_RS00860 Protein ID WP_013094943.1
Coordinates 173161..173565 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2J95_RS00840 169392..170930 + 1539 WP_013094940.1 aldehyde dehydrogenase -
B2J95_RS00845 170927..171892 - 966 WP_061771396.1 LysR family transcriptional regulator -
B2J95_RS00850 172010..172750 + 741 WP_013094942.1 MipA/OmpV family protein -
B2J95_RS00855 172961..173146 + 186 WP_071843252.1 type II toxin-antitoxin system HicA family toxin Toxin
B2J95_RS00860 173161..173565 + 405 WP_013094943.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
B2J95_RS00865 173619..175574 - 1956 WP_013094944.1 glycoside hydrolase family 127 protein -
B2J95_RS00870 175585..176985 - 1401 WP_038982539.1 MFS transporter -
B2J95_RS00875 177210..178028 + 819 WP_058661804.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6859.08 Da        Isoelectric Point: 11.7891

>T74028 WP_071843252.1 NZ_CP020089:172961-173146 [Enterobacter cloacae]
VKSADVITVLVRHGWKCVRTKGSHHQFRHPVHAGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Download         Length: 186 bp

>T74028 NZ_CP020089:172961-173146 [Enterobacter cloacae]
GTGAAAAGTGCGGATGTCATTACTGTTCTGGTCAGGCATGGCTGGAAATGTGTAAGAACGAAAGGAAGCCATCATCAGTT
TCGCCACCCAGTACATGCGGGGTTGGTGACCGTTCCACATCCCAAAAAGGACATAAAACCCGGCACCCTCGCGCAAATCT
GGCGGCAGGCGGGAATCAAACACTGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14915.74 Da        Isoelectric Point: 4.3036

>AT74028 WP_013094943.1 NZ_CP020089:173161-173565 [Enterobacter cloacae]
MFYPAYIHSDPDGSASGFFPDVPGCFFAGNSLDDAFQDARDALTAHFEALFEMDEELPLPGNVEAHLEATPGDFIGGQWL
LVDINMKQFDGKAERINITLPRRLLVKIDSFVNEHPQFSNRSAFLAEAARRVLP

Download         Length: 405 bp

>AT74028 NZ_CP020089:173161-173565 [Enterobacter cloacae]
ATGTTTTATCCGGCTTACATTCATTCTGATCCCGACGGTAGCGCCAGCGGTTTTTTTCCTGACGTTCCTGGTTGTTTTTT
TGCCGGCAATTCTCTGGATGATGCTTTTCAGGATGCACGTGATGCACTGACAGCTCACTTCGAAGCGCTATTTGAAATGG
ATGAAGAGTTACCTCTCCCTGGCAATGTTGAAGCACATCTTGAAGCCACACCGGGTGATTTTATCGGCGGACAATGGTTG
CTTGTAGATATCAATATGAAGCAATTCGACGGTAAGGCCGAGCGTATCAATATCACCCTGCCTCGTCGATTGCTGGTGAA
AATTGATTCTTTTGTGAATGAGCATCCGCAGTTTAGTAACCGAAGCGCTTTTCTTGCTGAGGCAGCGCGTCGCGTGCTGC
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A421IJD5

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z1F6W0

References