Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 185302..186059 Replicon plasmid unnamed1
Accession NZ_CP020087
Organism Shigella flexneri 1a strain 0670

Toxin (Protein)


Gene name tacT Uniprot ID D2AJG5
Locus tag B4376_RS26400 Protein ID WP_000501974.1
Coordinates 185302..185787 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag B4376_RS26405 Protein ID WP_011114751.1
Coordinates 185775..186059 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4376_RS26370 (B4376_26520) 180826..181250 - 425 Protein_220 hypothetical protein -
B4376_RS26375 (B4376_26525) 181478..182116 - 639 WP_000502854.1 ParB N-terminal domain-containing protein -
B4376_RS26380 182101..182244 - 144 Protein_222 DUF3440 domain-containing protein -
B4376_RS26395 (B4376_26540) 184848..185129 + 282 Protein_225 IS4/IS5 family transposase -
B4376_RS26400 (B4376_26545) 185302..185787 - 486 WP_000501974.1 GNAT family N-acetyltransferase Toxin
B4376_RS26405 (B4376_26550) 185775..186059 - 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
B4376_RS28045 186961..187125 + 165 WP_001346193.1 hypothetical protein -
B4376_RS26425 (B4376_26570) 187379..188980 - 1602 WP_005063916.1 IS66-like element ISSfl3 family transposase -
B4376_RS26430 (B4376_26575) 188977..189327 - 351 WP_005048975.1 IS66 family insertion sequence element accessory protein TnpB -
B4376_RS26435 (B4376_26580) 189324..189998 - 675 WP_004967157.1 IS66-like element accessory protein TnpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - spa29 / spa40 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 1..228834 228834
- inside IScluster/Tn - ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospC3 152546..198741 46195


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17814.63 Da        Isoelectric Point: 9.8822

>T74026 WP_000501974.1 NZ_CP020087:c185787-185302 [Shigella flexneri 1a]
MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T74026 NZ_CP020087:c185787-185302 [Shigella flexneri 1a]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCAGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGAGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT74026 WP_011114751.1 NZ_CP020087:c186059-185775 [Shigella flexneri 1a]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT74026 NZ_CP020087:c186059-185775 [Shigella flexneri 1a]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PN28

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References