Detailed information of TA system TA074025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 138185..138960
• Replicon: plasmid unnamed1
• Accession: NZ_CP020087
• Organism: Shigella flexneri 1a strain 0670

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q7BEG9
• Locus tag: B4376_RS26120
• Protein ID: WP_000405245.1
• Coordinates: 138185..138667 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: B4376_RS26125
• Protein ID: WP_010921625.1
• Coordinates: 138658..138960 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4376_RS26105 (B4376_26245)	134600..136291	+	1692	WP_094092434.1	T3SS effector E3 ubiquitin-protein ligase IpaH2.5	-
B4376_RS26110 (B4376_26250)	136343..137551	+	1209	Protein_174	IS3 family transposase	-
B4376_RS26115 (B4376_26255)	137594..138117	-	524	Protein_175	IS3-like element ISSfl11 family transposase	-
B4376_RS26120 (B4376_26260)	138185..138667	-	483	WP_000405245.1	GNAT family N-acetyltransferase	Toxin
B4376_RS26125 (B4376_26265)	138658..138960	-	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
B4376_RS26135 (B4376_26275)	139232..139504	+	273	WP_000019158.1	hypothetical protein	-
B4376_RS26140 (B4376_26280)	139485..140693	+	1209	WP_000198552.1	IS91 family transposase	-
B4376_RS28040	140865..140993	+	129	Protein_180	IS5/IS1182 family transposase	-
B4376_RS26150 (B4376_26290)	141142..142596	-	1455	WP_000701108.1	type 3 secretion system effector OspC2	-
B4376_RS26155 (B4376_26295)	143000..143619	+	620	Protein_182	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	spa29 / spa40 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24	1..228834	228834
	-	inside	IScluster/Tn	-	ospE2 / ipaH2.5 / ospC2	125489..145978	20489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17750.52 Da
• Isoelectric Point: 7.0382

>T74025 WP_000405245.1 NZ_CP020087:c138667-138185 [Shigella flexneri 1a]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T74025 NZ_CP020087:c138667-138185 [Shigella flexneri 1a]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTATTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT74025 WP_010921625.1 NZ_CP020087:c138960-138658 [Shigella flexneri 1a]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT74025 NZ_CP020087:c138960-138658 [Shigella flexneri 1a]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: Q7BEG9

Antitoxin

• AlphaFold DB: Q7BEG8