Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1056501..1057081 Replicon chromosome
Accession NZ_CP020085
Organism Glaesserella parasuis strain CL120103

Toxin (Protein)


Gene name mazF Uniprot ID A0A143CFE3
Locus tag B4X03_RS05285 Protein ID WP_005710599.1
Coordinates 1056501..1056845 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U4SAI4
Locus tag B4X03_RS05290 Protein ID WP_005710601.1
Coordinates 1056842..1057081 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4X03_RS05265 1051744..1052040 + 297 WP_021116569.1 DUF5374 domain-containing protein -
B4X03_RS05270 1052245..1054350 + 2106 WP_082259157.1 glycoside hydrolase family 2 TIM barrel-domain containing protein -
B4X03_RS05275 1054423..1055814 - 1392 WP_021116571.1 MATE family efflux transporter -
B4X03_RS05280 1055827..1056495 - 669 WP_021113308.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
B4X03_RS05285 1056501..1056845 - 345 WP_005710599.1 endoribonuclease MazF Toxin
B4X03_RS05290 1056842..1057081 - 240 WP_005710601.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
B4X03_RS05295 1057267..1058142 + 876 WP_021119465.1 putative selenate ABC transporter substrate-binding protein -
B4X03_RS05300 1058254..1059003 + 750 WP_005710605.1 phosphonate ABC transporter ATP-binding protein -
B4X03_RS05305 1058993..1059778 + 786 WP_016528266.1 ABC transporter permease subunit -
B4X03_RS05310 1059775..1060623 + 849 WP_075592852.1 ABC transporter permease subunit -
B4X03_RS05315 1060694..1061386 + 693 WP_021114597.1 tellurite resistance TerB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12830.81 Da        Isoelectric Point: 7.9277

>T73994 WP_005710599.1 NZ_CP020085:c1056845-1056501 [Glaesserella parasuis]
MTTQEYIPDVGDIIWLDFDPQAGHEQAGHRPALVLTPTIYNRQTGLLICCPLTTKVKGYPFEVNIAGTPQNVVLSDQIKS
LDWRIRHAKFKGKINHHQLSEVKAKIATLLQISE

Download         Length: 345 bp

>T73994 NZ_CP020085:c1056845-1056501 [Glaesserella parasuis]
ATGACTACTCAAGAGTATATCCCTGACGTTGGTGATATTATTTGGCTTGATTTTGATCCGCAAGCAGGACATGAACAAGC
TGGCCATCGTCCAGCATTAGTGTTAACGCCCACGATTTATAATCGTCAAACAGGGTTGTTAATTTGCTGCCCGCTCACAA
CAAAAGTAAAAGGCTATCCTTTCGAAGTCAATATTGCAGGTACCCCCCAAAATGTTGTGCTTTCCGATCAAATTAAAAGC
CTTGACTGGCGTATTCGTCACGCTAAATTTAAAGGTAAAATTAACCATCATCAGTTAAGCGAAGTAAAAGCTAAAATTGC
GACTTTGCTACAAATCAGTGAATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8782.21 Da        Isoelectric Point: 5.0351

>AT73994 WP_005710601.1 NZ_CP020085:c1057081-1056842 [Glaesserella parasuis]
MQVAVKKWGNSVGIRIPALLSNSLKLQAESLVDIQEVDGRLIIEPIKPKYNLEQLLSGITEDNLHNEVDFGKPVGKEML

Download         Length: 240 bp

>AT73994 NZ_CP020085:c1057081-1056842 [Glaesserella parasuis]
ATGCAAGTGGCTGTCAAAAAATGGGGAAATAGCGTAGGCATTCGCATTCCTGCTTTATTATCTAATTCATTGAAATTACA
GGCAGAGAGTTTAGTCGATATTCAAGAAGTTGATGGTCGCCTAATTATTGAGCCGATTAAACCTAAATATAATTTAGAGC
AACTTCTTTCAGGTATTACTGAAGATAATTTGCACAATGAAGTCGATTTTGGCAAGCCTGTTGGCAAGGAAATGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A143CFE3

Antitoxin

Source ID Structure
AlphaFold DB U4SAI4

References