Detailed information of TA system TA073990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 131278..132965
• Replicon: chromosome
• Accession: NZ_CP020085
• Organism: Glaesserella parasuis strain CL120103

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U4SB19
• Locus tag: B4X03_RS00730
• Protein ID: WP_021111224.1
• Coordinates: 131622..132965 (-)
• Length: 448 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: B4X03_RS00725
• Protein ID: WP_223300026.1
• Coordinates: 131278..131640 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4X03_RS00705	127346..128296	-	951	WP_005713153.1	ribose-phosphate pyrophosphokinase	-
B4X03_RS00710	128324..129169	-	846	WP_021113204.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
B4X03_RS00715	129171..129803	-	633	WP_021110345.1	lipoprotein insertase outer membrane protein LolB	-
B4X03_RS00720	129924..131249	-	1326	WP_082259028.1	ATP-dependent RNA helicase SrmB	-
B4X03_RS00725	131278..131640	-	363	WP_223300026.1	helix-turn-helix transcriptional regulator	Antitoxin
B4X03_RS00730	131622..132965	-	1344	WP_021111224.1	HipA domain-containing protein	Toxin
B4X03_RS00735	133079..133516	-	438	WP_005713142.1	ribosomal protein S18-alanine N-acetyltransferase	-
B4X03_RS00740	133690..133917	+	228	Protein_138	transposase	-
B4X03_RS00745	133997..135676	-	1680	WP_005713398.1	DNA repair protein RecN	-
B4X03_RS00750	135919..136144	-	226	Protein_140	hypothetical protein	-
B4X03_RS00755	136154..136996	+	843	WP_021114212.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	133666..133917	251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (161-407)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-98)

Sequences

Toxin

Protein

• Length: 448 a.a.
• Molecular weight: 51106.20 Da
• Isoelectric Point: 5.4867

>T73990 WP_021111224.1 NZ_CP020085:c132965-131622 [Glaesserella parasuis]
MQIEPLILQAYIADRWQDIAILTFQADYSLNDFSYLHDLTEILSDNDKAVSFNYPVEFFPHRSVSGWFAFLDDIVPSGAS
RRYWLQKLDIAHLPMAQQNYLLLKQGTIAPVGHLRVKQAVSSFVENEPSRLFEIDDVINRQADFLEYANQRGAMAGGATG
AGGEAPKLLLRQTATRQVWIDNLQQGASSDDFYLVKFPRGKRSVIDCDILRAEFHYYHELASMGFDTIDTTKMRLEEGER
YPSLWLPRFDVYRNEQGKMARYAVESVYAMLEKGAGSLLDHEKVIRQLIAKIETSHLVETGMFFDKQAFVIEWVRRDLLN
IAFGNSDNHGRNTAFLRNQNRIWLAPIYDFAPMKADPEGIPRTTMWDSKGHPALELGGEYRFDLIAEQLDDLCPADKLLD
ALRQTAQQLVGLKARLEARGVPTAILTYPSISLDYLPEKLKRWQLLS

Nucleotide

• Length: 1344 bp

>T73990 NZ_CP020085:c132965-131622 [Glaesserella parasuis]
ATGCAAATCGAACCGCTTATACTTCAAGCCTACATTGCTGATCGTTGGCAGGATATTGCTATTTTGACCTTTCAGGCTGA
TTATAGTCTCAATGATTTTAGCTATCTCCATGATTTAACAGAAATTTTAAGTGATAATGATAAGGCAGTTTCATTTAACT
ACCCTGTGGAATTTTTTCCGCATCGCAGTGTATCGGGCTGGTTTGCATTTTTAGATGATATAGTCCCTTCTGGGGCAAGC
CGGCGTTATTGGTTGCAAAAACTGGATATTGCTCATTTGCCGATGGCTCAACAAAACTATTTGTTATTAAAACAAGGCAC
GATTGCACCTGTCGGGCATTTACGAGTTAAACAAGCGGTCAGTTCTTTTGTCGAAAATGAACCCTCTCGCTTATTTGAAA
TAGACGATGTTATCAATCGCCAAGCAGACTTTTTGGAATACGCTAATCAGCGTGGGGCAATGGCTGGAGGTGCCACAGGG
GCTGGCGGTGAAGCACCGAAATTATTGCTACGCCAAACAGCCACTCGGCAAGTTTGGATTGATAACCTACAACAAGGTGC
CTCTTCTGACGATTTCTATTTAGTCAAGTTTCCGCGTGGGAAACGTAGTGTAATAGATTGCGATATATTGAGAGCTGAAT
TTCACTATTATCACGAATTAGCCTCAATGGGTTTTGACACGATTGATACGACAAAAATGCGATTGGAAGAAGGCGAACGT
TACCCGTCGTTGTGGTTACCTCGTTTTGATGTTTATCGTAATGAACAGGGGAAAATGGCTCGTTATGCCGTTGAATCTGT
TTATGCTATGTTAGAAAAAGGGGCTGGCTCTTTGCTGGATCACGAAAAGGTTATTCGTCAGTTGATTGCTAAAATTGAAA
CCTCTCATTTGGTTGAAACAGGAATGTTTTTTGATAAACAAGCTTTTGTGATCGAGTGGGTAAGGCGAGATTTATTGAAT
ATCGCCTTTGGAAATAGCGATAATCACGGGCGAAATACGGCTTTCTTACGCAATCAAAATCGTATTTGGCTTGCTCCAAT
TTATGATTTTGCCCCAATGAAAGCAGATCCTGAAGGTATTCCTCGCACGACAATGTGGGATAGTAAAGGTCACCCTGCAT
TAGAATTAGGCGGTGAATATCGTTTTGATCTGATTGCAGAACAGCTTGATGATTTATGTCCAGCCGACAAGTTATTGGAT
GCTTTGCGTCAAACGGCTCAACAGCTTGTCGGACTTAAAGCACGTTTAGAAGCAAGGGGCGTTCCAACGGCGATTTTGAC
GTATCCTTCAATCAGTTTGGACTATTTACCTGAAAAATTAAAACGTTGGCAATTATTATCATAA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13880.42 Da
• Isoelectric Point: 10.2782

>AT73990 WP_223300026.1 NZ_CP020085:c131640-131278 [Glaesserella parasuis]
MAIIIIMEEIKQARASKSKKTLDIYQRATVRDEIARKLLLNEMSLGQALKYLRLHLLAMKQERYAEIVKVSRKTLSDLEN
DKGNYSIDIINQVLRPFELQLGVVPMNKTLLRQVLNEQAV

Nucleotide

• Length: 363 bp

>AT73990 NZ_CP020085:c131640-131278 [Glaesserella parasuis]
TTGGCAATTATTATCATAATGGAAGAGATCAAACAGGCGAGAGCCTCTAAAAGTAAAAAAACGTTAGATATTTATCAGAG
AGCGACTGTGCGAGACGAAATTGCTCGTAAGCTATTGCTCAATGAGATGAGTTTAGGGCAGGCGTTGAAATATCTACGTT
TACATCTACTGGCAATGAAACAAGAGCGTTATGCGGAGATTGTCAAAGTTTCTCGCAAAACGTTATCTGATTTAGAAAAT
GATAAGGGAAATTACAGTATTGATATTATCAATCAGGTGTTACGCCCCTTTGAATTACAACTAGGTGTTGTGCCAATGAA
CAAAACACTACTCAGACAAGTTTTAAACGAACAAGCGGTCTGA

Structures

Toxin

• AlphaFold DB: U4SB19