Detailed information of TA system TA073989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 33463..34102
• Replicon: chromosome
• Accession: NZ_CP020085
• Organism: Glaesserella parasuis strain CL120103

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6I4QYS7
• Locus tag: B4X03_RS00225
• Protein ID: WP_021114433.1
• Coordinates: 33463..33798 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: B4X03_RS00230
• Protein ID: WP_164681833.1
• Coordinates: 33806..34102 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4X03_RS00190	29191..29520	-	330	WP_021113605.1	hypothetical protein	-
B4X03_RS00195	29662..30234	+	573	WP_021116087.1	sigma-70 family RNA polymerase sigma factor	-
B4X03_RS00200	30231..30416	+	186	WP_005713975.1	zf-HC2 domain-containing protein	-
B4X03_RS00205	30605..31349	-	745	Protein_40	IS481 family transposase	-
B4X03_RS00210	31441..32379	-	939	WP_062923993.1	hypothetical protein	-
B4X03_RS00215	32456..32785	-	330	WP_082259016.1	hypothetical protein	-
B4X03_RS00220	32900..33304	-	405	WP_021110905.1	DNA polymerase III subunit psi	-
B4X03_RS00225	33463..33798	+	336	WP_021114433.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B4X03_RS00230	33806..34102	+	297	WP_164681833.1	putative addiction module antidote protein	Antitoxin
B4X03_RS00235	34175..35164	+	990	WP_021114432.1	16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC	-
B4X03_RS00240	35265..36390	+	1126	Protein_47	IS3 family transposase	-
B4X03_RS00245	36417..36967	-	551	Protein_48	IS5 family transposase	-
B4X03_RS11385	37148..37711	+	564	WP_236933856.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	23739..36967	13228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-96)

Antitoxin

• Uncharacterized protein HI1420 (9-91)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12079.77 Da
• Isoelectric Point: 9.8168

>T73989 WP_021114433.1 NZ_CP020085:33463-33798 [Glaesserella parasuis]
MSGIINQIVITDNFSKWLDGLKNPIAKATVARRIKNAVKGNFGDHKALAGTGGLYEMRIATGAGYRIYYAQQGDVIYILL
QGGDKSTQQADIEKAKALWAELKQTGGSNEY

Nucleotide

• Length: 336 bp

>T73989 NZ_CP020085:33463-33798 [Glaesserella parasuis]
ATGTCAGGAATTATCAACCAAATCGTCATTACAGATAATTTTTCAAAGTGGCTAGATGGTTTAAAAAATCCTATTGCTAA
AGCAACAGTGGCAAGAAGAATTAAGAATGCAGTAAAAGGTAATTTTGGTGATCATAAAGCGCTTGCAGGTACTGGTGGAC
TTTATGAAATGCGAATAGCGACAGGCGCTGGTTATCGAATTTACTATGCACAGCAGGGCGATGTTATTTATATTCTGTTA
CAAGGTGGTGATAAATCAACACAGCAAGCCGATATTGAGAAAGCGAAAGCATTGTGGGCAGAATTAAAACAGACAGGAGG
AAGTAATGAATATTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10948.49 Da
• Isoelectric Point: 4.8951

>AT73989 WP_164681833.1 NZ_CP020085:33806-34102 [Glaesserella parasuis]
MAEKHGLRPFDVAEFLDNEETIQAYLAEVLKEGDQDEFLEALNDVARARGIAELAKQTGLGRESLYKTLSKGSKPRFETI
QKILGAFNLVLIPQIKNS

Nucleotide

• Length: 297 bp

>AT73989 NZ_CP020085:33806-34102 [Glaesserella parasuis]
ATGGCAGAAAAACACGGTTTACGTCCTTTTGATGTCGCTGAATTTTTGGATAACGAAGAAACAATACAAGCCTATTTAGC
AGAAGTATTAAAAGAGGGCGATCAAGATGAGTTTTTGGAAGCCCTTAATGATGTAGCAAGAGCAAGGGGAATCGCAGAAC
TTGCGAAGCAAACGGGGTTAGGTAGAGAAAGTTTATATAAAACCCTATCTAAAGGAAGTAAACCTCGTTTTGAAACTATT
CAAAAAATTTTAGGAGCATTTAATCTTGTGCTTATCCCACAAATAAAAAATAGTTAA

Structures

Toxin

• AlphaFold DB: A0A6I4QYS7