Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 9393..10036 Replicon plasmid tig00000003
Accession NZ_CP020073
Organism Klebsiella pneumoniae strain AR_0115

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag AM362_RS28340 Protein ID WP_000754566.1
Coordinates 9620..10036 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag AM362_RS28335 Protein ID WP_001261276.1
Coordinates 9393..9623 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM362_RS28300 4512..4781 + 270 WP_000339857.1 hypothetical protein -
AM362_RS28305 4958..5824 - 867 WP_004118283.1 replication initiation protein -
AM362_RS29990 6354..6458 + 105 WP_032409716.1 hypothetical protein -
AM362_RS28310 6587..6844 + 258 WP_000764642.1 hypothetical protein -
AM362_RS28315 6902..7678 - 777 WP_000015958.1 site-specific integrase -
AM362_RS28320 7675..8418 - 744 WP_000129823.1 hypothetical protein -
AM362_RS28325 8469..8819 - 351 WP_000493378.1 hypothetical protein -
AM362_RS28335 9393..9623 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM362_RS28340 9620..10036 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
AM362_RS28345 10240..11096 + 857 Protein_13 IS3-like element ISEc15 family transposase -
AM362_RS28355 11378..12454 + 1077 WP_000227969.1 IS110 family transposase -
AM362_RS29320 13095..13178 + 84 Protein_15 SOS response-associated peptidase -
AM362_RS28365 13341..14198 - 858 Protein_16 Tn3 family transposase -
AM362_RS28370 14284..15036 + 753 WP_000602738.1 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(4)-Ia / aac(3)-IVa / sul3 / ant(3'')-Ia / cmlA1 / aadA2 - 1..39762 39762


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T73981 WP_000754566.1 NZ_CP020073:9620-10036 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T73981 NZ_CP020073:9620-10036 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT73981 WP_001261276.1 NZ_CP020073:9393-9623 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT73981 NZ_CP020073:9393-9623 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References