Detailed information of TA system TA073961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 17687..18330
• Replicon: plasmid unitig_4
• Accession: NZ_CP020070
• Organism: Klebsiella pneumoniae strain AR_0068

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: AM447_RS29435
• Protein ID: WP_001044770.1
• Coordinates: 17914..18330 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: AM447_RS29430
• Protein ID: WP_001261282.1
• Coordinates: 17687..17917 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM447_RS29400	13259..14125	-	867	WP_004118283.1	replication initiation protein	-
AM447_RS31310	14660..14764	+	105	WP_032409716.1	hypothetical protein	-
AM447_RS29405	14893..15150	+	258	WP_000764642.1	hypothetical protein	-
AM447_RS29410	15208..15984	-	777	WP_000015958.1	site-specific integrase	-
AM447_RS29415	15981..16724	-	744	WP_000129823.1	hypothetical protein	-
AM447_RS29420	16775..17125	-	351	WP_000493378.1	hypothetical protein	-
AM447_RS29425	17269..17730	-	462	WP_072202616.1	hypothetical protein	-
AM447_RS29430	17687..17917	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AM447_RS29435	17914..18330	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM447_RS29440	18404..19114	+	711	WP_004152334.1	ATP-binding protein	-
AM447_RS29445	19148..19845	+	698	Protein_25	IS1 family transposase	-
AM447_RS29450	19930..20790	-	861	WP_000027057.1	class A broad-spectrum beta-lactamase TEM-1	-
AM447_RS29455	20973..21290	-	318	Protein_27	recombinase family protein	-
AM447_RS29460	21490..22314	-	825	WP_000722315.1	oxacillin-hydrolyzing class D beta-lactamase OXA-9	-
AM447_RS29465	22374..23162	-	789	WP_001206315.1	ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaCTX-M-15	-	1..37875	37875

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T73961 WP_001044770.1 NZ_CP020070:17914-18330 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T73961 NZ_CP020070:17914-18330 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT73961 WP_001261282.1 NZ_CP020070:17687-17917 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT73961 NZ_CP020070:17687-17917 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3