Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 28284..28806 Replicon plasmid unitig_1
Accession NZ_CP020068
Organism Klebsiella pneumoniae strain AR_0068

Toxin (Protein)


Gene name stbE Uniprot ID A0A0C7KG00
Locus tag AM447_RS26695 Protein ID WP_038991638.1
Coordinates 28522..28806 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag AM447_RS26690 Protein ID WP_004181777.1
Coordinates 28284..28532 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM447_RS26670 23493..24314 - 822 WP_004181772.1 hypothetical protein -
AM447_RS26675 24376..24729 - 354 WP_004181774.1 hypothetical protein -
AM447_RS26680 24874..25860 - 987 WP_040120328.1 hypothetical protein -
AM447_RS26685 26194..27993 - 1800 WP_043907032.1 ATP-dependent helicase -
AM447_RS26690 28284..28532 + 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AM447_RS26695 28522..28806 + 285 WP_038991638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM447_RS30070 28823..28924 - 102 Protein_30 IS200/IS605 family transposase -
AM447_RS26700 28960..30186 + 1227 WP_080030933.1 transposase -
AM447_RS26705 30458..30682 - 225 Protein_32 transposase -
AM447_RS26710 30761..31189 - 429 WP_077256229.1 IS200/IS605 family transposase -
AM447_RS26715 31225..32412 + 1188 WP_043907031.1 transposase -
AM447_RS30075 32511..33606 - 1096 Protein_35 conjugal transfer protein TraG N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / blaSHV-187 / aac(3)-IId / blaNDM-1 / aph(3')-VI / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 - 1..276460 276460


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10993.73 Da        Isoelectric Point: 10.6516

>T73957 WP_038991638.1 NZ_CP020068:28522-28806 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Download         Length: 285 bp

>T73957 NZ_CP020068:28522-28806 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT73957 WP_004181777.1 NZ_CP020068:28284-28532 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT73957 NZ_CP020068:28284-28532 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7KG00

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References