Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4329651..4330276 Replicon chromosome
Accession NZ_CP020061
Organism Klebsiella pneumoniae strain AR_0117

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag AM364_RS21115 Protein ID WP_002882817.1
Coordinates 4329651..4330034 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag AM364_RS21120 Protein ID WP_004150355.1
Coordinates 4330034..4330276 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM364_RS21100 4327017..4327919 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
AM364_RS21105 4327916..4328551 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
AM364_RS21110 4328548..4329477 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
AM364_RS21115 4329651..4330034 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
AM364_RS21120 4330034..4330276 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
AM364_RS21125 4330481..4331398 + 918 WP_020802762.1 alpha/beta hydrolase -
AM364_RS21130 4331412..4332353 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
AM364_RS21135 4332398..4332835 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
AM364_RS21140 4332832..4333692 - 861 WP_004146232.1 virulence factor BrkB family protein -
AM364_RS21145 4333686..4334285 - 600 WP_004146231.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T73935 WP_002882817.1 NZ_CP020061:c4330034-4329651 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T73935 NZ_CP020061:c4330034-4329651 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT73935 WP_004150355.1 NZ_CP020061:c4330276-4330034 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT73935 NZ_CP020061:c4330276-4330034 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References