Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3085602..3086221 Replicon chromosome
Accession NZ_CP020061
Organism Klebsiella pneumoniae strain AR_0117

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AM364_RS15025 Protein ID WP_002892050.1
Coordinates 3086003..3086221 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag AM364_RS15020 Protein ID WP_002892066.1
Coordinates 3085602..3085976 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM364_RS15010 3080754..3081947 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM364_RS15015 3081970..3085116 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
AM364_RS15020 3085602..3085976 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
AM364_RS15025 3086003..3086221 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AM364_RS15030 3086380..3086817 + 438 Protein_2880 maltose O-acetyltransferase -
AM364_RS15035 3086913..3088121 + 1209 WP_001339197.1 IS4-like element ISVsa5 family transposase -
AM364_RS15040 3088135..3088284 + 150 Protein_2882 maltose O-acetyltransferase -
AM364_RS29905 3088256..3088396 - 141 WP_004147370.1 hypothetical protein -
AM364_RS15045 3088417..3088887 + 471 WP_020802585.1 YlaC family protein -
AM364_RS15050 3088862..3090313 - 1452 WP_020802627.1 PLP-dependent aminotransferase family protein -
AM364_RS15055 3090414..3091112 + 699 WP_002892021.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3086913..3088121 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T73931 WP_002892050.1 NZ_CP020061:3086003-3086221 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T73931 NZ_CP020061:3086003-3086221 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT73931 WP_002892066.1 NZ_CP020061:3085602-3085976 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT73931 NZ_CP020061:3085602-3085976 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References