Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 31510..32032 Replicon plasmid unitig_2
Accession NZ_CP020060
Organism Escherichia coli strain AR_0061

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag AM440_RS24305 Protein ID WP_000220560.1
Coordinates 31751..32032 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag AM440_RS24300 Protein ID WP_000121743.1
Coordinates 31510..31761 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM440_RS24270 27181..27462 + 282 WP_001185482.1 hypothetical protein -
AM440_RS24275 27487..28464 + 978 WP_024245156.1 hypothetical protein -
AM440_RS24280 28461..28886 - 426 WP_000202942.1 hypothetical protein -
AM440_RS24285 28954..29400 - 447 Protein_34 hypothetical protein -
AM440_RS24290 29440..30276 - 837 WP_001050931.1 replication initiation protein -
AM440_RS24300 31510..31761 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
AM440_RS24305 31751..32032 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM440_RS24315 32107..32804 + 698 Protein_38 IS1 family transposase -
AM440_RS24320 32798..33403 - 606 WP_080025760.1 IS6 family transposase -
AM440_RS25150 33415..33555 - 141 WP_020219413.1 hypothetical protein -
AM440_RS24330 33719..34276 + 558 WP_001235713.1 recombinase family protein -
AM440_RS24335 34459..35319 + 861 WP_000027057.1 class A broad-spectrum beta-lactamase TEM-1 -
AM440_RS24340 35577..36368 - 792 WP_000034420.1 sulfonamide-resistant dihydropteroate synthase Sul3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / blaTEM-1B / sul3 / sul1 / qacE / aadA2 / dfrA12 - 1..50999 50999


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T73919 WP_000220560.1 NZ_CP020060:31751-32032 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T73919 NZ_CP020060:31751-32032 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT73919 WP_000121743.1 NZ_CP020060:31510-31761 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT73919 NZ_CP020060:31510-31761 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References