Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3839738..3840392 Replicon chromosome
Accession NZ_CP020058
Organism Escherichia coli strain AR_0061

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag AM440_RS19785 Protein ID WP_000244777.1
Coordinates 3839985..3840392 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AM440_RS19780 Protein ID WP_000354046.1
Coordinates 3839738..3840004 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM440_RS19760 3835707..3837140 - 1434 WP_001514525.1 6-phospho-beta-glucosidase BglA -
AM440_RS19765 3837185..3837496 + 312 WP_001514524.1 N(4)-acetylcytidine aminohydrolase -
AM440_RS19770 3837660..3838319 + 660 WP_000250274.1 hemolysin III family protein -
AM440_RS19775 3838515..3839495 - 981 WP_080025700.1 tRNA-modifying protein YgfZ -
AM440_RS19780 3839738..3840004 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AM440_RS19785 3839985..3840392 + 408 WP_000244777.1 toxin CptA Toxin
AM440_RS19790 3840432..3840953 - 522 WP_080025701.1 flavodoxin FldB -
AM440_RS19795 3841065..3841961 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AM440_RS19800 3841986..3842696 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AM440_RS19805 3842702..3844435 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T73917 WP_000244777.1 NZ_CP020058:3839985-3840392 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T73917 NZ_CP020058:3839985-3840392 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT73917 WP_000354046.1 NZ_CP020058:3839738-3840004 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT73917 NZ_CP020058:3839738-3840004 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References