Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1724830..1725448 Replicon chromosome
Accession NZ_CP020058
Organism Escherichia coli strain AR_0061

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AM440_RS08870 Protein ID WP_001291435.1
Coordinates 1725230..1725448 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AM440_RS08865 Protein ID WP_000344800.1
Coordinates 1724830..1725204 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM440_RS08855 1719919..1721112 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM440_RS08860 1721135..1724284 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
AM440_RS08865 1724830..1725204 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AM440_RS08870 1725230..1725448 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AM440_RS08875 1725619..1726170 + 552 WP_000102564.1 maltose O-acetyltransferase -
AM440_RS08880 1726286..1726756 + 471 WP_000136192.1 YlaC family protein -
AM440_RS08885 1726920..1728470 + 1551 WP_001514985.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AM440_RS08890 1728512..1728865 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AM440_RS08900 1729244..1729555 + 312 WP_162780194.1 MGMT family protein -
AM440_RS08905 1729586..1730158 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T73907 WP_001291435.1 NZ_CP020058:1725230-1725448 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T73907 NZ_CP020058:1725230-1725448 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT73907 WP_000344800.1 NZ_CP020058:1724830-1725204 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT73907 NZ_CP020058:1724830-1725204 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References