Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3267418..3268036 Replicon chromosome
Accession NZ_CP020055
Organism Escherichia coli strain AR_0069

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AM448_RS16725 Protein ID WP_001291435.1
Coordinates 3267418..3267636 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AM448_RS16730 Protein ID WP_000344800.1
Coordinates 3267662..3268036 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM448_RS16690 3262707..3263279 + 573 WP_000779831.1 YbaY family lipoprotein -
AM448_RS16695 3263310..3263621 - 312 WP_000409911.1 MGMT family protein -
AM448_RS16705 3264000..3264353 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AM448_RS16710 3264395..3265945 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AM448_RS16715 3266109..3266579 - 471 WP_000136192.1 YlaC family protein -
AM448_RS16720 3266695..3267246 - 552 WP_080028831.1 maltose O-acetyltransferase -
AM448_RS16725 3267418..3267636 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AM448_RS16730 3267662..3268036 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AM448_RS16735 3268582..3271731 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
AM448_RS16740 3271754..3272947 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T73894 WP_001291435.1 NZ_CP020055:c3267636-3267418 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T73894 NZ_CP020055:c3267636-3267418 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT73894 WP_000344800.1 NZ_CP020055:c3268036-3267662 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT73894 NZ_CP020055:c3268036-3267662 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References