Detailed information of TA system TA073891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 1754939..1755739
• Replicon: chromosome
• Accession: NZ_CP020055
• Organism: Escherichia coli strain AR_0069

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: AM448_RS09075
• Protein ID: WP_080028757.1
• Coordinates: 1754939..1755466 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: AM448_RS09080
• Protein ID: WP_001277108.1
• Coordinates: 1755473..1755739 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM448_RS09045	1750014..1750781	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
AM448_RS09050	1750778..1752055	-	1278	WP_000803799.1	branched chain amino acid ABC transporter permease LivM	-
AM448_RS09055	1752052..1752978	-	927	WP_000003004.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
AM448_RS09060	1753026..1754135	-	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
AM448_RS09070	1754559..1754942	+	384	WP_000778781.1	aspartate 1-decarboxylase autocleavage activator PanM	-
AM448_RS09075	1754939..1755466	-	528	WP_080028757.1	GNAT family N-acetyltransferase	Toxin
AM448_RS09080	1755473..1755739	-	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
AM448_RS09085	1755889..1756992	-	1104	WP_001350438.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
AM448_RS09095	1757264..1758118	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
AM448_RS09100	1758363..1759421	-	1059	WP_001042003.1	permease-like cell division protein FtsX	-
AM448_RS09105	1759414..1760082	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19719.75 Da
• Isoelectric Point: 7.7457

>T73891 WP_080028757.1 NZ_CP020055:c1755466-1754939 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRVYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T73891 NZ_CP020055:c1755466-1754939 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGTGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT73891 WP_001277108.1 NZ_CP020055:c1755739-1755473 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT73891 NZ_CP020055:c1755739-1755473 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• PDB: 6GTS
• PDB: 6AJN
• PDB: 6GTQ
• PDB: 6GTO
• PDB: 6GTR
• PDB: 6AJM
• AlphaFold DB: A0A829CN24