Detailed information of TA system TA073868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2566008..2566624
• Replicon: chromosome
• Accession: NZ_CP020053
• Organism: Enterobacter cloacae complex sp. strain AR_0065

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AM444_RS12620
• Protein ID: WP_023295637.1
• Coordinates: 2566253..2566624 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: AM444_RS12615
• Protein ID: WP_015569912.1
• Coordinates: 2566008..2566250 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM444_RS12585 (AM444_12690)	2561067..2561867	+	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-
AM444_RS12590 (AM444_12695)	2561986..2562585	+	600	WP_003861947.1	glucose-1-phosphatase	-
AM444_RS12595 (AM444_12700)	2562579..2563460	+	882	WP_003861949.1	virulence factor BrkB family protein	-
AM444_RS12600 (AM444_12705)	2563457..2563894	+	438	WP_023295639.1	D-aminoacyl-tRNA deacylase	-
AM444_RS12605 (AM444_12710)	2563939..2564880	+	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
AM444_RS12610 (AM444_12715)	2564889..2565809	-	921	WP_023295638.1	alpha/beta hydrolase	-
AM444_RS12615 (AM444_12720)	2566008..2566250	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
AM444_RS12620 (AM444_12725)	2566253..2566624	+	372	WP_023295637.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM444_RS12625 (AM444_12730)	2566664..2567593	-	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
AM444_RS12630 (AM444_12735)	2567590..2568225	-	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
AM444_RS12635 (AM444_12740)	2568222..2569124	-	903	WP_014072386.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13751.90 Da
• Isoelectric Point: 6.4882

>T73868 WP_023295637.1 NZ_CP020053:2566253-2566624 [Enterobacter cloacae complex sp.]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIVERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T73868 NZ_CP020053:2566253-2566624 [Enterobacter cloacae complex sp.]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGTGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT73868 WP_015569912.1 NZ_CP020053:2566008-2566250 [Enterobacter cloacae complex sp.]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT73868 NZ_CP020053:2566008-2566250 [Enterobacter cloacae complex sp.]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAACTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3