Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1834573..1835149 Replicon chromosome
Accession NZ_CP020053
Organism Enterobacter cloacae complex sp. strain AR_0065

Toxin (Protein)


Gene name shpA Uniprot ID A0A800YKM1
Locus tag AM444_RS09050 Protein ID WP_015572580.1
Coordinates 1834862..1835149 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A801DSF4
Locus tag AM444_RS09045 Protein ID WP_017694570.1
Coordinates 1834573..1834875 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM444_RS09025 (AM444_09110) 1830868..1831413 + 546 WP_006810254.1 YfaZ family outer membrane protein -
AM444_RS09030 (AM444_09115) 1831591..1832961 - 1371 WP_017694568.1 NAD-dependent succinate-semialdehyde dehydrogenase -
AM444_RS09035 (AM444_09120) 1833115..1833867 + 753 WP_015572582.1 AraC family transcriptional regulator -
AM444_RS09040 (AM444_09125) 1833906..1834544 + 639 WP_032673114.1 LysE family translocator -
AM444_RS09045 (AM444_09130) 1834573..1834875 - 303 WP_017694570.1 BrnA antitoxin family protein Antitoxin
AM444_RS09050 (AM444_09135) 1834862..1835149 - 288 WP_015572580.1 BrnT family toxin Toxin
AM444_RS09055 (AM444_09140) 1835319..1836590 + 1272 WP_015572579.1 DUF445 domain-containing protein -
AM444_RS09060 (AM444_09145) 1836587..1837663 - 1077 WP_015572578.1 DUF2955 domain-containing protein -
AM444_RS09065 (AM444_09150) 1837653..1838720 - 1068 WP_086524236.1 HlyD family secretion protein -
AM444_RS09070 (AM444_09155) 1838717..1839187 - 471 WP_015572576.1 MarR family transcriptional regulator -
AM444_RS09075 (AM444_09160) 1839337..1840056 - 720 WP_015572575.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11296.81 Da        Isoelectric Point: 7.3587

>T73865 WP_015572580.1 NZ_CP020053:c1835149-1834862 [Enterobacter cloacae complex sp.]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Download         Length: 288 bp

>T73865 NZ_CP020053:c1835149-1834862 [Enterobacter cloacae complex sp.]
ATGCCTATAGAGTATGAATGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGTGTTATTGTCATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11482.13 Da        Isoelectric Point: 10.4094

>AT73865 WP_017694570.1 NZ_CP020053:c1834875-1834573 [Enterobacter cloacae complex sp.]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT73865 NZ_CP020053:c1834875-1834573 [Enterobacter cloacae complex sp.]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGTGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References