Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 61532..62271 Replicon chromosome
Accession NZ_CP020053
Organism Enterobacter cloacae complex sp. strain AR_0065

Toxin (Protein)


Gene name tacT Uniprot ID A0A3L9PBP9
Locus tag AM444_RS00300 Protein ID WP_003857133.1
Coordinates 61786..62271 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag AM444_RS00295 Protein ID WP_003857131.1
Coordinates 61532..61798 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM444_RS00275 (AM444_00275) 57008..57994 + 987 WP_023296632.1 DUF979 domain-containing protein -
AM444_RS00280 (AM444_00280) 58004..58999 + 996 WP_015570513.1 DUF2891 domain-containing protein -
AM444_RS00285 (AM444_00285) 59021..60409 - 1389 WP_032103005.1 MFS transporter -
AM444_RS00290 (AM444_00290) 60539..61468 + 930 WP_023296630.1 LysR family transcriptional regulator -
AM444_RS00295 (AM444_00295) 61532..61798 + 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
AM444_RS00300 (AM444_00300) 61786..62271 + 486 WP_003857133.1 GNAT family N-acetyltransferase Toxin
AM444_RS00305 (AM444_00305) 62442..63734 - 1293 WP_023296629.1 glycosyl hydrolase family 28 protein -
AM444_RS00310 (AM444_00310) 63839..64597 - 759 WP_015570520.1 DeoR/GlpR family DNA-binding transcription regulator -
AM444_RS00315 (AM444_00315) 64684..65268 + 585 WP_017382349.1 GDP-mannose pyrophosphatase nudK -
AM444_RS00320 (AM444_00320) 65353..66111 + 759 WP_023296628.1 trans-aconitate 2-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(58-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17540.23 Da        Isoelectric Point: 9.9658

>T73863 WP_003857133.1 NZ_CP020053:61786-62271 [Enterobacter cloacae complex sp.]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T73863 NZ_CP020053:61786-62271 [Enterobacter cloacae complex sp.]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTTAGTGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGGAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATACCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGAGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTCAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCCGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT73863 WP_003857131.1 NZ_CP020053:61532-61798 [Enterobacter cloacae complex sp.]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT73863 NZ_CP020053:61532-61798 [Enterobacter cloacae complex sp.]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACAGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCACAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3L9PBP9

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References