Detailed information of TA system TA073861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2064688..2065709
• Replicon: chromosome
• Accession: NZ_CP020052
• Organism: Proteus mirabilis strain AR_0059

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A8E4FRK4
• Locus tag: AM438_RS09520
• Protein ID: WP_017827532.1
• Coordinates: 2064688..2065257 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: AM438_RS09525
• Protein ID: WP_004245528.1
• Coordinates: 2065257..2065709 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM438_RS09515	2062218..2064572	-	2355	WP_049212337.1	DNA polymerase II	-
AM438_RS09520	2064688..2065257	-	570	WP_017827532.1	PIN domain-containing protein	Toxin
AM438_RS09525	2065257..2065709	-	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
AM438_RS09530	2065837..2066538	-	702	WP_049212340.1	thiamine ABC transporter ATP-binding protein ThiQ	-
AM438_RS09535	2066531..2068111	-	1581	WP_004245526.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
AM438_RS09540	2068111..2069124	-	1014	WP_004250228.1	thiamine ABC transporter substrate binding subunit	-
AM438_RS09545	2069393..2069923	+	531	WP_004245524.1	RNA pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21601.08 Da
• Isoelectric Point: 7.0180

>T73861 WP_017827532.1 NZ_CP020052:c2065257-2064688 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKDPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T73861 NZ_CP020052:c2065257-2064688 [Proteus mirabilis]
ATGACAAACTATACTGTTTTGCTTGATGCCTGTGTCATGTATCCAGCCCCTCTTAGAAGTTATTTAATGTATCTTGCTAA
TACTGGTTTATTTAGAGCACGTTGGAGTAATCAGATCCATGATGAATGGATCACTAATTTATTAGTCAACAATCCAACAT
TAACAGCTGAGCGACTAATACGAACCAGAGATTTAATGAATGCACATGTTCCTGATTGTTTAGTAGAAGGCTACGAACCG
CTTATTTGTGGTTTAAAGCTACCTGATCCTAATGATCGTCATGTGCTAGCAGCAGCAATAAAAGGGCAGGCTCAAGCTAT
AATTACATTTAACTTAAAAGATTTTCCGCAAAAATACCTTAATTCTTTTGATATTTCAGCTATCCATCCTGATGAATTTT
TGAGTGACATGTTTGAATTAAATCCTAGTAGCTGTATTAAAGCGGCGCAACAACATAGACAAAGCCTAAAGGATCCACCA
ATGACCGTAGAGACCTTTTTAGGCTGTTTACAAAAGCAGAAATTACCCCAGTTTATTTCCCATTTACGGGCATTCCAGAT
ATTATTATAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT73861 WP_004245528.1 NZ_CP020052:c2065709-2065257 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT73861 NZ_CP020052:c2065709-2065257 [Proteus mirabilis]
ATGAATACGATAAGTGAGAGACTTAGTGCTGAAGATATTGCATTAGCAAAATTAAGTAGCCAAGAGCTATCCGCTGTTAT
GGATATTAATGGCAAAACGCAGAAGCTATCCATTATAGATAAGACGGGTAATAATCATATTGTACAAATACCAAATAGTG
CATTACGCCTAATGATTGAAATTCTTACTCAACTAGGGCAAGGGAATACTGTTAGTATCACACCAATCCATGCTGAGCTA
ACGACACAGGAAGCGGCTGATATACTCAATATGTCCCGTCCTACATTTGTAAAATTATTAGATACACGAGCGATACCCTA
CTCTAGAAAAGGAAATCGTAGGAAAGTTGCTTATGCTGATATTGTTGCTTATAAAAATGAATTGGAGCAAAAACGACTTG
ATGCCCTTGCTGAATTATCGCAATTAGATCAAGAAATGGATATGGGGTATTAA

Structures

Antitoxin

• AlphaFold DB: B4F2H3