Detailed information of TA system TA073858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 65906..66507
• Replicon: plasmid unitig_3
• Accession: NZ_CP020051
• Organism: Escherichia coli strain AR_0118

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: AM365_RS28125
• Protein ID: WP_069067274.1
• Coordinates: 66127..66507 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: AM365_RS28120
• Protein ID: WP_001190712.1
• Coordinates: 65906..66127 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM365_RS28070	61116..61805	+	690	WP_069067277.1	hypothetical protein	-
AM365_RS28075	61792..62478	+	687	WP_080028522.1	ead/Ea22-like family protein	-
AM365_RS28080	62475..63188	+	714	WP_069067276.1	ead/Ea22-like family protein	-
AM365_RS28980	63188..63364	+	177	WP_001261535.1	hypothetical protein	-
AM365_RS28085	63361..63549	+	189	WP_000797282.1	hypothetical protein	-
AM365_RS28090	63551..63760	+	210	WP_000951710.1	hypothetical protein	-
AM365_RS28095	63757..64458	+	702	WP_074446721.1	hypothetical protein	-
AM365_RS28100	64442..64726	+	285	WP_001142396.1	hypothetical protein	-
AM365_RS28105	64711..64920	-	210	WP_001344848.1	hypothetical protein	-
AM365_RS28110	65094..65345	+	252	WP_032220968.1	DNA polymerase III subunit theta	-
AM365_RS28115	65444..65833	+	390	WP_069067275.1	DNA repair protein	-
AM365_RS28120	65906..66127	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AM365_RS28125	66127..66507	+	381	WP_069067274.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AM365_RS28130	66591..67391	-	801	WP_000432092.1	hypothetical protein	-
AM365_RS28135	67398..68060	-	663	WP_069067273.1	DUF2829 domain-containing protein	-
AM365_RS28140	68075..68569	-	495	WP_000640903.1	dUTP diphosphatase	-
AM365_RS28145	68566..69042	-	477	WP_000861173.1	hypothetical protein	-
AM365_RS28150	69039..69347	-	309	WP_000349257.1	hypothetical protein	-
AM365_RS28155	69457..70485	-	1029	WP_080028523.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91291	91291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13607.51 Da
• Isoelectric Point: 5.6406

>T73858 WP_069067274.1 NZ_CP020051:66127-66507 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGKAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Nucleotide

• Length: 381 bp

>T73858 NZ_CP020051:66127-66507 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAAGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTTCCAATATTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT73858 WP_001190712.1 NZ_CP020051:65906-66127 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT73858 NZ_CP020051:65906-66127 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6