Detailed information of TA system TA073845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3968111..3968736
• Replicon: chromosome
• Accession: NZ_CP020048
• Organism: Escherichia coli strain AR_0118

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AM365_RS20645
• Protein ID: WP_000911330.1
• Coordinates: 3968338..3968736 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AM365_RS20640
• Protein ID: WP_000450524.1
• Coordinates: 3968111..3968338 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM365_RS20615	3963914..3964384	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
AM365_RS20620	3964384..3964956	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
AM365_RS20625	3965102..3965980	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AM365_RS20630	3965997..3967031	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
AM365_RS20635	3967244..3967957	+	714	WP_001347624.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AM365_RS20640	3968111..3968338	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AM365_RS20645	3968338..3968736	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AM365_RS20650	3968883..3969746	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
AM365_RS20655	3969761..3971776	+	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
AM365_RS20660	3971850..3972548	+	699	WP_000679812.1	esterase	-
AM365_RS20665	3972629..3972829	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T73845 WP_000911330.1 NZ_CP020048:3968338-3968736 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T73845 NZ_CP020048:3968338-3968736 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT73845 WP_000450524.1 NZ_CP020048:3968111-3968338 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT73845 NZ_CP020048:3968111-3968338 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33