Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3524241..3524895 Replicon chromosome
Accession NZ_CP020048
Organism Escherichia coli strain AR_0118

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag AM365_RS18420 Protein ID WP_000244772.1
Coordinates 3524488..3524895 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AM365_RS18415 Protein ID WP_000354046.1
Coordinates 3524241..3524507 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM365_RS18390 3519529..3520272 + 744 WP_000951964.1 SDR family oxidoreductase -
AM365_RS18395 3520329..3521762 - 1434 WP_024198262.1 6-phospho-beta-glucosidase BglA -
AM365_RS18400 3521807..3522118 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AM365_RS18405 3522282..3522941 + 660 WP_000250274.1 hemolysin III family protein -
AM365_RS18410 3523018..3523998 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AM365_RS18415 3524241..3524507 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AM365_RS18420 3524488..3524895 + 408 WP_000244772.1 protein YgfX Toxin
AM365_RS18425 3524935..3525456 - 522 WP_001055874.1 flavodoxin FldB -
AM365_RS18430 3525568..3526464 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AM365_RS18435 3526489..3527199 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AM365_RS18440 3527205..3528938 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T73843 WP_000244772.1 NZ_CP020048:3524488-3524895 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T73843 NZ_CP020048:3524488-3524895 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT73843 WP_000354046.1 NZ_CP020048:3524241-3524507 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT73843 NZ_CP020048:3524241-3524507 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References