Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2945972..2946603 Replicon chromosome
Accession NZ_CP020048
Organism Escherichia coli strain AR_0118

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag AM365_RS15300 Protein ID WP_001259388.1
Coordinates 2945972..2946247 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AM365_RS15305 Protein ID WP_000593555.1
Coordinates 2946244..2946603 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM365_RS15285 2940976..2942043 + 1068 WP_000361485.1 HlyD family secretion protein -
AM365_RS15290 2942040..2944775 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
AM365_RS15295 2944775..2945899 + 1125 WP_001314210.1 ABC transporter permease -
AM365_RS15300 2945972..2946247 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
AM365_RS15305 2946244..2946603 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AM365_RS15310 2946723..2947124 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
AM365_RS15315 2947130..2947936 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
AM365_RS15320 2947933..2948697 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
AM365_RS15325 2948697..2949530 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
AM365_RS15330 2949527..2950471 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T73841 WP_001259388.1 NZ_CP020048:2945972-2946247 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T73841 NZ_CP020048:2945972-2946247 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT73841 WP_000593555.1 NZ_CP020048:2946244-2946603 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT73841 NZ_CP020048:2946244-2946603 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References