Detailed information of TA system TA073838

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2141136..2141971
• Replicon: chromosome
• Accession: NZ_CP020048
• Organism: Escherichia coli strain AR_0118

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9IW37
• Locus tag: AM365_RS11255
• Protein ID: WP_023356554.1
• Coordinates: 2141594..2141971 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9IW59
• Locus tag: AM365_RS11250
• Protein ID: WP_001360327.1
• Coordinates: 2141136..2141504 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM365_RS11210	2136800..2137480	+	681	WP_023356552.1	WYL domain-containing protein	-
AM365_RS11215	2137628..2138305	+	678	WP_001097302.1	hypothetical protein	-
AM365_RS11220	2138311..2138544	+	234	WP_001278293.1	DUF905 family protein	-
AM365_RS11225	2138634..2139452	+	819	WP_001175165.1	DUF945 domain-containing protein	-
AM365_RS11230	2139718..2140197	+	480	WP_000706978.1	antirestriction protein	-
AM365_RS11235	2140213..2140689	+	477	WP_001186774.1	RadC family protein	-
AM365_RS11240	2140752..2140973	+	222	WP_023356553.1	DUF987 domain-containing protein	-
AM365_RS11250	2141136..2141504	+	369	WP_001360327.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM365_RS11255	2141594..2141971	+	378	WP_023356554.1	TA system toxin CbtA family protein	Toxin
AM365_RS28485	2141968..2142117	+	150	Protein_2065	hypothetical protein	-
AM365_RS28490	2142196..2142390	+	195	WP_024181941.1	DUF957 domain-containing protein	-
AM365_RS11265	2142475..2143317	+	843	WP_022645115.1	DUF4942 domain-containing protein	-
AM365_RS11275	2144066..2145603	+	1538	Protein_2068	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2101056..2153416	52360

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14052.01 Da
• Isoelectric Point: 7.8045

>T73838 WP_023356554.1 NZ_CP020048:2141594-2141971 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFADERVIEQHIEAGIALCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T73838 NZ_CP020048:2141594-2141971 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTGCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.34 Da
• Isoelectric Point: 5.9598

>AT73838 WP_001360327.1 NZ_CP020048:2141136-2141504 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT73838 NZ_CP020048:2141136-2141504 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9IW37

Antitoxin

• AlphaFold DB: A0A7U9IW59