Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1249924..1250542 Replicon chromosome
Accession NZ_CP020048
Organism Escherichia coli strain AR_0118

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AM365_RS06540 Protein ID WP_001291435.1
Coordinates 1250324..1250542 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AM365_RS06535 Protein ID WP_000344800.1
Coordinates 1249924..1250298 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM365_RS06525 1245013..1246206 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM365_RS06530 1246229..1249378 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
AM365_RS06535 1249924..1250298 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AM365_RS06540 1250324..1250542 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AM365_RS06545 1250713..1251264 + 552 WP_000102564.1 maltose O-acetyltransferase -
AM365_RS06550 1251380..1251850 + 471 WP_000136192.1 YlaC family protein -
AM365_RS06555 1252014..1253564 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AM365_RS06560 1253606..1253959 - 354 WP_000878141.1 DUF1428 family protein -
AM365_RS06570 1254338..1254649 + 312 WP_000409911.1 MGMT family protein -
AM365_RS06575 1254680..1255252 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T73834 WP_001291435.1 NZ_CP020048:1250324-1250542 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T73834 NZ_CP020048:1250324-1250542 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT73834 WP_000344800.1 NZ_CP020048:1249924-1250298 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT73834 NZ_CP020048:1249924-1250298 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References