Detailed information of TA system TA073830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 589853..590524
• Replicon: chromosome
• Accession: NZ_CP020046
• Organism: Thiomonas intermedia strain ATCC 15466

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BVH73_RS02675
• Protein ID: WP_079415852.1
• Coordinates: 590108..590524 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BVH73_RS02670
• Protein ID: WP_079415850.1
• Coordinates: 589853..590104 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVH73_RS02645	586685..587395	-	711	WP_079415840.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
BVH73_RS02650	587388..587939	-	552	WP_079415842.1	helix-turn-helix domain-containing protein	-
BVH73_RS02655	588058..588384	+	327	WP_079415844.1	ATP-dependent helicase	-
BVH73_RS02660	588428..588844	-	417	WP_079415846.1	hypothetical protein	-
BVH73_RS02665	588822..589451	-	630	WP_079415848.1	nucleotidyltransferase domain-containing protein	-
BVH73_RS02670	589853..590104	+	252	WP_079415850.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BVH73_RS02675	590108..590524	+	417	WP_079415852.1	type II toxin-antitoxin system VapC family toxin	Toxin
BVH73_RS02680	590607..590843	+	237	WP_079415854.1	hypothetical protein	-
BVH73_RS02685	590993..592273	+	1281	WP_154048516.1	UDP-N-acetylglucosamine 2-epimerase (hydrolyzing)	-
BVH73_RS02690	592294..592944	+	651	WP_079415856.1	NeuD/PglB/VioB family sugar acetyltransferase	-
BVH73_RS02695	593189..593404	+	216	WP_079420261.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BVH73_RS02700	593427..593840	+	414	WP_079415858.1	type II toxin-antitoxin system VapC family toxin	-
BVH73_RS02705	593875..595455	+	1581	WP_079415860.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15333.87 Da
• Isoelectric Point: 8.5684

>T73830 WP_079415852.1 NZ_CP020046:590108-590524 [Thiomonas intermedia]
VSYLVDTNVLSELRRREPAPAVVQWMEARPTTALYLSVLTVGELRKGIEAMTAGLRKQQLLDWLDVELPAFFANRLLPVD
LKVAERWGKLLSQGGRPLPAIDSLLAATALTHDLTLVTRNVRDFQFSGLAVIDPWAAK

Nucleotide

• Length: 417 bp

>T73830 NZ_CP020046:590108-590524 [Thiomonas intermedia]
GTGAGCTACCTCGTCGATACCAACGTCCTGTCCGAACTGCGACGTCGCGAACCGGCACCAGCGGTTGTGCAGTGGATGGA
AGCGCGCCCGACCACAGCCTTGTATCTGTCTGTCCTGACCGTGGGCGAATTGCGCAAAGGCATCGAGGCGATGACCGCTG
GCCTGCGCAAACAACAACTGCTCGACTGGCTGGATGTTGAATTGCCTGCGTTCTTCGCAAACCGGCTGCTCCCGGTCGAT
CTGAAGGTCGCCGAACGCTGGGGAAAATTGCTGTCACAAGGCGGGCGCCCTCTTCCCGCCATCGACAGCCTGCTTGCCGC
TACCGCCCTGACCCATGATCTGACCCTCGTGACTCGCAATGTGCGTGACTTTCAGTTTTCAGGGCTAGCGGTCATCGACC
CTTGGGCCGCCAAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9336.44 Da
• Isoelectric Point: 5.1255

>AT73830 WP_079415850.1 NZ_CP020046:589853-590104 [Thiomonas intermedia]
MHIWQMQEAKSRLSEVVKEAERDGPQEITVHGRSVAVVLSRAEYDRLAGTGESLASFIRRSPLANAEDLDFPRDTSLTRE
NPL

Nucleotide

• Length: 252 bp

>AT73830 NZ_CP020046:589853-590104 [Thiomonas intermedia]
ATGCACATCTGGCAAATGCAAGAAGCTAAATCGCGCCTTTCCGAGGTGGTCAAAGAGGCCGAGCGCGACGGCCCGCAGGA
AATCACGGTACATGGCAGGTCGGTGGCCGTCGTGCTGTCGCGCGCCGAGTATGATCGTTTGGCCGGTACCGGTGAGTCAC
TGGCTTCCTTTATACGCCGCTCGCCGTTGGCGAATGCAGAGGATCTCGATTTCCCGCGTGACACCAGCCTCACGCGGGAA
AACCCCCTGTAA