Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 251756..252392 Replicon chromosome
Accession NZ_CP020030
Organism Geobacillus thermodenitrificans strain T12

Toxin (Protein)


Gene name mazF Uniprot ID L7ZVP1
Locus tag GTHT12_RS01325 Protein ID WP_003253417.1
Coordinates 252042..252392 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0Q1ENP8
Locus tag GTHT12_RS01320 Protein ID WP_008881474.1
Coordinates 251756..252037 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTHT12_RS01300 247939..248556 - 618 WP_060475533.1 rhomboid family intramembrane serine protease -
GTHT12_RS01305 248697..249086 + 390 WP_008881477.1 holo-ACP synthase -
GTHT12_RS01310 249166..250179 + 1014 WP_008881476.1 outer membrane lipoprotein carrier protein LolA -
GTHT12_RS01315 250474..251628 + 1155 WP_060475534.1 alanine racemase -
GTHT12_RS01320 251756..252037 + 282 WP_008881474.1 hypothetical protein Antitoxin
GTHT12_RS01325 252042..252392 + 351 WP_003253417.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GTHT12_RS01330 253053..255218 + 2166 WP_029761551.1 RNA-binding transcriptional accessory protein -
GTHT12_RS01335 255235..255348 - 114 WP_087959817.1 cortex morphogenetic protein CmpA -
GTHT12_RS01340 255439..255927 + 489 WP_008881471.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12992.00 Da        Isoelectric Point: 4.8781

>T73822 WP_003253417.1 NZ_CP020030:252042-252392 [Geobacillus thermodenitrificans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T73822 NZ_CP020030:252042-252392 [Geobacillus thermodenitrificans]
TTGATTGTTAAACGTGGCGACGTTTATTTTGCGGACCTTTCCCCGGTCGTTGGCTCAGAGCAGGGCGGCGTACGCCCCGT
GTTGGTGATCCAAAACGATATCGGTAATCGCTTTAGTCCGACGGTGATTGTGGCGGCGATTACGGCGCAAATCCAGAAGG
CGAAACTGCCGACGCATGTTGAAATCGATGCAAAACGCTACGGGTTTGAGCGCGATTCCGTCATTTTGCTTGAGCAAATT
CGTACGATTGATAAGCAACGGTTGACCGATAAAATTACCCACTTGGACGATGAAATGATGGATAAAGTTGATGAGGCGCT
GCAAATCAGCTTAGGATTGATCGACTTTTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10627.09 Da        Isoelectric Point: 7.2027

>AT73822 WP_008881474.1 NZ_CP020030:251756-252037 [Geobacillus thermodenitrificans]
VSESGATTEIVVRLPQSLLTELDGLVKQENGNRNELIYQATKMYIRERKKRQIREAMRRGYMEMAKINLSIASEAFHAEY
EADHTVERLVSGG

Download         Length: 282 bp

>AT73822 NZ_CP020030:251756-252037 [Geobacillus thermodenitrificans]
GTGTCGGAATCTGGCGCAACAACGGAAATCGTCGTTCGTTTGCCGCAGTCGCTGCTGACGGAACTGGACGGGCTTGTCAA
ACAGGAGAACGGCAATCGAAATGAACTCATTTATCAAGCGACGAAAATGTATATTCGTGAACGGAAAAAACGGCAAATTC
GCGAGGCGATGAGACGAGGCTACATGGAAATGGCCAAAATCAATTTATCTATTGCTTCTGAAGCGTTTCATGCCGAATAT
GAGGCCGACCACACCGTTGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9ERQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0Q1ENP8

References