Detailed information of TA system TA073821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 4206058..4206666
• Replicon: chromosome
• Accession: NZ_CP020028
• Organism: Paenibacillus kribbensis strain AM49

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: B4V02_RS18755
• Protein ID: WP_007429335.1
• Coordinates: 4206058..4206408 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: B4V02_RS18760
• Protein ID: WP_170970806.1
• Coordinates: 4206415..4206666 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4V02_RS18725	4201543..4202424	+	882	WP_094155922.1	Cof-type HAD-IIB family hydrolase	-
B4V02_RS18740	4202819..4203307	-	489	WP_094155923.1	SprT family protein	-
B4V02_RS18745	4203451..4203585	+	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
B4V02_RS18750	4203656..4205878	-	2223	WP_094155924.1	RNA-binding transcriptional accessory protein	-
B4V02_RS18755	4206058..4206408	-	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B4V02_RS18760	4206415..4206666	-	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
B4V02_RS18765	4206990..4208186	-	1197	WP_094155925.1	alanine racemase	-
B4V02_RS18770	4208474..4209748	-	1275	WP_094155926.1	outer membrane lipoprotein carrier protein LolA	-
B4V02_RS18775	4209873..4210505	-	633	WP_094155927.1	LysE family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T73821 WP_007429335.1 NZ_CP020028:c4206408-4206058 [Paenibacillus kribbensis]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T73821 NZ_CP020028:c4206408-4206058 [Paenibacillus kribbensis]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCAGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTCAGTCCTACCGTGATCGTGGCAGCCATCACCGCCCAGATTCAGAAGG
CAAAGCTGCCCACGCATGTGGAGATCGACGCGGCAACGCATGGCTTTGATCGCGATTCTGTCGTTCTTCTGGAGCAAATT
CGGACGATCGACAAACAAAGGCTCACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
TCAAATCAGTCTTGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT73821 WP_170970806.1 NZ_CP020028:c4206666-4206415 [Paenibacillus kribbensis]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT73821 NZ_CP020028:c4206666-4206415 [Paenibacillus kribbensis]
ATGATCAGTTTGCCGGATCATCTCTTGCAGGAAGTGGATGGGATCGTAGCTATGGAAAATTCCAACCGTAGTGAATTTAT
TAGGCAGGCCATGAAGCTGTATGTAGGCGAACGTAAAAAACGTTACATTCGTGAAGCGATGCAGCGTGGGTACATGGAAA
TGGCTAAAATTAACTTGACCATGGCATCCGAAGCCTTTCACGCGGAGGAAGATGCTAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1