Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3667843..3668497 Replicon chromosome
Accession NZ_CP020025
Organism Escherichia coli strain WB61

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag B1T56_RS18445 Protein ID WP_000244781.1
Coordinates 3667843..3668250 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag B1T56_RS18450 Protein ID WP_000354046.1
Coordinates 3668231..3668497 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1T56_RS18425 3663800..3665533 - 1734 WP_077516661.1 single-stranded-DNA-specific exonuclease RecJ -
B1T56_RS18430 3665539..3666249 - 711 WP_077516663.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
B1T56_RS18435 3666274..3667170 - 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
B1T56_RS18440 3667282..3667803 + 522 WP_032303736.1 flavodoxin FldB -
B1T56_RS18445 3667843..3668250 - 408 WP_000244781.1 protein YgfX Toxin
B1T56_RS18450 3668231..3668497 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
B1T56_RS18455 3668740..3669720 + 981 WP_077516665.1 tRNA-modifying protein YgfZ -
B1T56_RS18460 3669797..3670456 - 660 WP_000250269.1 hemolysin III family protein -
B1T56_RS18465 3670620..3670931 - 312 WP_077516667.1 N(4)-acetylcytidine aminohydrolase -
B1T56_RS18470 3670976..3672409 + 1434 WP_136698981.1 6-phospho-beta-glucosidase BglA -
B1T56_RS18475 3672457..3672600 - 144 Protein_3426 transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T73815 WP_000244781.1 NZ_CP020025:c3668250-3667843 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T73815 NZ_CP020025:c3668250-3667843 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT73815 WP_000354046.1 NZ_CP020025:c3668497-3668231 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT73815 NZ_CP020025:c3668497-3668231 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References