Detailed information of TA system TA073812

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2723732..2724567
• Replicon: chromosome
• Accession: NZ_CP020025
• Organism: Escherichia coli strain WB61

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0H2VCF5
• Locus tag: B1T56_RS13955
• Protein ID: WP_000854681.1
• Coordinates: 2724190..2724567 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: B1T56_RS13950
• Protein ID: WP_105178465.1
• Coordinates: 2723732..2724100 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1T56_RS13925	2721007..2721462	+	456	WP_000581506.1	hypothetical protein	-
B1T56_RS23850	2721563..2721771	+	209	Protein_2573	DUF905 domain-containing protein	-
B1T56_RS13930	2721777..2722445	+	669	Protein_2574	DUF932 domain-containing protein	-
B1T56_RS24100	2722464..2722877	+	414	Protein_2575	antirestriction protein	-
B1T56_RS13940	2722893..2723369	+	477	WP_001354275.1	RadC family protein	-
B1T56_RS13945	2723432..2723653	+	222	WP_000692300.1	DUF987 domain-containing protein	-
B1T56_RS13950	2723732..2724100	+	369	WP_105178465.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B1T56_RS13955	2724190..2724567	+	378	WP_000854681.1	TA system toxin CbtA family protein	Toxin
B1T56_RS13960	2724564..2724713	+	150	Protein_2580	hypothetical protein	-
B1T56_RS13965	2724859..2725212	+	354	Protein_2581	EutP/PduV family microcompartment system protein	-
B1T56_RS13970	2725313..2725642	-	330	WP_000450409.1	DUF496 family protein	-
B1T56_RS13975	2725814..2726872	-	1059	WP_001200890.1	FUSC family protein	-
B1T56_RS13980	2727070..2727543	-	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
B1T56_RS13985	2727662..2728828	-	1167	WP_001303036.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13963.89 Da
• Isoelectric Point: 7.4348

>T73812 WP_000854681.1 NZ_CP020025:2724190-2724567 [Escherichia coli]
MKTLPDTHIREASHCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTSHNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T73812 NZ_CP020025:2724190-2724567 [Escherichia coli]
ATGAAAACATTACCCGACACACACATACGGGAGGCATCGCACTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAGAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCAGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13728.52 Da
• Isoelectric Point: 6.9465

>AT73812 WP_105178465.1 NZ_CP020025:2723732-2724100 [Escherichia coli]
VSDTLPGTTHPDNNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT73812 NZ_CP020025:2723732-2724100 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0H2VCF5