Detailed information of TA system TA073797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1556754..1557459
• Replicon: chromosome
• Accession: NZ_CP020025
• Organism: Escherichia coli strain WB61

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: B1T56_RS07925
• Protein ID: WP_000539521.1
• Coordinates: 1557073..1557459 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: B1T56_RS07920
• Protein ID: WP_001280945.1
• Coordinates: 1556754..1557083 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1T56_RS07905	1551912..1552823	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
B1T56_RS07910	1553000..1555348	+	2349	WP_022645413.1	EAL domain-containing protein	-
B1T56_RS07915	1555356..1556684	+	1329	WP_022645414.1	GGDEF domain-containing protein	-
B1T56_RS07920	1556754..1557083	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
B1T56_RS07925	1557073..1557459	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B1T56_RS07930	1557685..1559010	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
B1T56_RS07935	1559223..1559606	+	384	WP_000497137.1	biofilm formation regulator BssR	-
B1T56_RS07940	1559717..1560832	+	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
B1T56_RS07945	1560829..1561455	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T73797 WP_000539521.1 NZ_CP020025:c1557459-1557073 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T73797 NZ_CP020025:c1557459-1557073 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGTAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT73797 WP_001280945.1 NZ_CP020025:c1557083-1556754 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT73797 NZ_CP020025:c1557083-1556754 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6