Detailed information of TA system TA073791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 547846..548441
• Replicon: chromosome
• Accession: NZ_CP020025
• Organism: Escherichia coli strain WB61

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: B1T56_RS02815
• Protein ID: WP_000239581.1
• Coordinates: 548091..548441 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: B1T56_RS02810
• Protein ID: WP_001223213.1
• Coordinates: 547846..548097 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1T56_RS02800	543511..547290	+	3780	WP_022646473.1	autotransporter assembly complex protein TamB	-
B1T56_RS02805	547293..547634	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
B1T56_RS02810	547846..548097	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
B1T56_RS02815	548091..548441	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
B1T56_RS02820	548520..549050	-	531	WP_000055075.1	inorganic diphosphatase	-
B1T56_RS02825	549360..550316	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
B1T56_RS02830	550449..551951	+	1503	WP_022646474.1	sugar ABC transporter ATP-binding protein	-
B1T56_RS02835	551965..552987	+	1023	WP_001298067.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T73791 WP_000239581.1 NZ_CP020025:548091-548441 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T73791 NZ_CP020025:548091-548441 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT73791 WP_001223213.1 NZ_CP020025:547846-548097 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT73791 NZ_CP020025:547846-548097 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7