Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2788325..2788961 Replicon chromosome
Accession NZ_CP020023
Organism Bacillus subtilis strain ATCC 21228

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BS21228_RS14865 Protein ID WP_003156187.1
Coordinates 2788611..2788961 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag BS21228_RS14860 Protein ID WP_003225183.1
Coordinates 2788325..2788606 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS21228_RS14840 2784684..2785283 - 600 WP_014478896.1 rhomboid family intramembrane serine protease -
BS21228_RS14845 2785378..2785743 + 366 WP_014478897.1 holo-ACP synthase -
BS21228_RS14850 2785909..2786925 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
BS21228_RS14855 2787040..2788209 + 1170 WP_014478898.1 alanine racemase -
BS21228_RS14860 2788325..2788606 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BS21228_RS14865 2788611..2788961 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BS21228_RS14870 2789077..2789901 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
BS21228_RS14875 2789906..2790271 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
BS21228_RS14880 2790275..2790676 + 402 WP_014478899.1 serine/threonine-protein kinase RsbT -
BS21228_RS14885 2790688..2791695 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
BS21228_RS14890 2791764..2792093 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
BS21228_RS14895 2792090..2792572 + 483 WP_003234299.1 anti-sigma B factor RsbW -
BS21228_RS14900 2792538..2793326 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
BS21228_RS14905 2793326..2793925 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T73788 WP_003156187.1 NZ_CP020023:2788611-2788961 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T73788 NZ_CP020023:2788611-2788961 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT73788 WP_003225183.1 NZ_CP020023:2788325-2788606 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT73788 NZ_CP020023:2788325-2788606 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References