Detailed information of TA system TA073782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2100275..2100804
• Replicon: chromosome
• Accession: NZ_CP020020
• Organism: Staphylococcus aureus strain ATCC 6538

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B4602_RS10880
• Protein ID: WP_000621175.1
• Coordinates: 2100275..2100637 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: B4602_RS10885
• Protein ID: WP_000948331.1
• Coordinates: 2100634..2100804 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4602_RS10855	2097253..2098023	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
B4602_RS10860	2097998..2098477	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
B4602_RS10865	2098479..2098805	-	327	WP_001052491.1	anti-sigma factor antagonist	-
B4602_RS10870	2098924..2099925	-	1002	WP_042727554.1	PP2C family protein-serine/threonine phosphatase	-
B4602_RS10880	2100275..2100637	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B4602_RS10885	2100634..2100804	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
B4602_RS10890	2100889..2102037	-	1149	WP_001281154.1	alanine racemase	-
B4602_RS10895	2102103..2102462	-	360	WP_000581197.1	holo-ACP synthase	-
B4602_RS10900	2102466..2102957	-	492	WP_001205912.1	PH domain-containing protein	-
B4602_RS10905	2102944..2104527	-	1584	WP_001294620.1	PH domain-containing protein	-
B4602_RS10910	2104520..2104999	-	480	WP_001287079.1	hypothetical protein	-
B4602_RS10915	2105208..2105768	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T73782 WP_000621175.1 NZ_CP020020:c2100637-2100275 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T73782 NZ_CP020020:c2100637-2100275 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT73782 WP_000948331.1 NZ_CP020020:c2100804-2100634 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT73782 NZ_CP020020:c2100804-2100634 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1