Detailed information of TA system TA073770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2118621..2119150
• Replicon: chromosome
• Accession: NZ_CP020019
• Organism: Staphylococcus aureus strain 08S00974

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B4603_RS10845
• Protein ID: WP_000621175.1
• Coordinates: 2118621..2118983 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: B4603_RS10850
• Protein ID: WP_000948331.1
• Coordinates: 2118980..2119150 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4603_RS10820	2115599..2116369	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
B4603_RS10825	2116344..2116823	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
B4603_RS10830	2116825..2117151	-	327	WP_001052491.1	anti-sigma factor antagonist	-
B4603_RS10835	2117270..2118271	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
B4603_RS10845	2118621..2118983	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B4603_RS10850	2118980..2119150	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
B4603_RS10855	2119235..2120383	-	1149	WP_001281139.1	alanine racemase	-
B4603_RS10860	2120449..2120808	-	360	WP_000581197.1	holo-ACP synthase	-
B4603_RS10865	2120812..2121303	-	492	WP_001286980.1	PH domain-containing protein	-
B4603_RS10870	2121296..2122873	-	1578	WP_001294650.1	PH domain-containing protein	-
B4603_RS10875	2122866..2123345	-	480	WP_001287081.1	hypothetical protein	-
B4603_RS10880	2123554..2124114	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T73770 WP_000621175.1 NZ_CP020019:c2118983-2118621 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T73770 NZ_CP020019:c2118983-2118621 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT73770 WP_000948331.1 NZ_CP020019:c2119150-2118980 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT73770 NZ_CP020019:c2119150-2118980 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1