73763

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1771468..1772032 Replicon chromosome
Accession NZ_CP020017
Organism Tetragenococcus halophilus strain KUD23

Toxin (Protein)


Gene name mazF Uniprot ID A0A2H6CPR8
Locus tag B2J85_RS08470 Protein ID WP_014123850.1
Coordinates 1771468..1771803 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3G5FHJ2
Locus tag B2J85_RS08475 Protein ID WP_041591760.1
Coordinates 1771796..1772032 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2J85_RS08440 1766757..1768226 - 1470 WP_061840057.1 betaine-aldehyde dehydrogenase -
B2J85_RS08445 1768469..1769029 + 561 WP_014123854.1 hypothetical protein -
B2J85_RS08450 1769079..1769942 + 864 WP_094244182.1 glycine betaine ABC transporter substrate-binding protein -
B2J85_RS12900 1770211..1770578 - 368 Protein_1659 zeta toxin family protein -
B2J85_RS08465 1770643..1771194 - 552 WP_094243844.1 GNAT family N-acetyltransferase -
B2J85_RS08470 1771468..1771803 - 336 WP_014123850.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
B2J85_RS08475 1771796..1772032 - 237 WP_041591760.1 hypothetical protein Antitoxin
B2J85_RS08480 1772535..1773952 - 1418 Protein_1663 ISLre2 family transposase -
B2J85_RS08485 1774153..1774215 + 63 Protein_1664 hypothetical protein -
B2J85_RS08490 1774270..1774749 + 480 WP_094243845.1 UTRA domain-containing protein -
B2J85_RS08495 1775018..1775654 - 637 Protein_1666 IS110 family transposase -
B2J85_RS08500 1775920..1776612 - 693 WP_005225225.1 Crp/Fnr family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1772567..1773952 1385


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12890.98 Da        Isoelectric Point: 8.9118

>T73763 WP_014123850.1 NZ_CP020017:c1771803-1771468 [Tetragenococcus halophilus]
MTNHYTPEKGDIVWIDFDPSTGMEIQKRRPALVISRGNFNTATKFAVVCPITSTEIDLPTRYSLSENYKTKGQIVIHQMK
SLDFNKRKIDFIEKMNPVDLKKIEQIIEFII

Download         Length: 336 bp

>T73763 NZ_CP020017:c1771803-1771468 [Tetragenococcus halophilus]
ATGACTAATCACTATACACCAGAAAAAGGAGACATAGTGTGGATCGACTTTGATCCTTCAACTGGAATGGAAATTCAAAA
GCGACGGCCAGCTCTGGTAATTTCGCGCGGAAATTTTAATACGGCGACAAAATTTGCGGTGGTATGTCCTATTACATCCA
CTGAAATAGATTTACCAACTAGATATAGTCTTTCAGAAAATTATAAAACAAAAGGACAAATAGTCATCCATCAAATGAAA
TCTTTAGATTTCAATAAACGCAAAATTGACTTTATTGAGAAAATGAACCCTGTGGACTTGAAGAAAATAGAACAGATCAT
TGAGTTCATTATTTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8506.60 Da        Isoelectric Point: 4.0338

>AT73763 WP_041591760.1 NZ_CP020017:c1772032-1771796 [Tetragenococcus halophilus]
MLKTKTRAQGRSIVVTLPAEDGATVFPGKEYLVSYGNDGTIVLIPKIEDPFANVTEGAFYESDVWEDMPAVGKEVLDD

Download         Length: 237 bp

>AT73763 NZ_CP020017:c1772032-1771796 [Tetragenococcus halophilus]
ATGCTAAAGACAAAAACAAGGGCACAAGGTAGATCTATCGTGGTTACTTTACCAGCTGAGGATGGGGCAACAGTTTTTCC
TGGGAAAGAATATCTTGTTTCATACGGAAATGATGGAACTATTGTTTTGATACCTAAAATTGAAGATCCGTTTGCTAATG
TTACAGAAGGAGCTTTTTACGAATCGGATGTATGGGAAGATATGCCAGCAGTTGGTAAAGAGGTGCTGGATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H6CPR8

Antitoxin

Source ID Structure
AlphaFold DB A0A3G5FHJ2

References