Detailed information of TA system TA073701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1080270..1080901
• Replicon: chromosome
• Accession: NZ_CP020007
• Organism: Haemophilus influenzae strain 48P153H1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q4QLW0
• Locus tag: BV085_RS05415
• Protein ID: WP_005651560.1
• Coordinates: 1080270..1080668 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BV085_RS05420
• Protein ID: WP_005686209.1
• Coordinates: 1080668..1080901 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BV085_RS05395	1075463..1076581	+	1119	WP_005686201.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-
BV085_RS05400	1076582..1077604	+	1023	WP_005655937.1	outer membrane-stress sensor serine endopeptidase DegS	-
BV085_RS05405	1077678..1078493	-	816	WP_005686204.1	DNA-formamidopyrimidine glycosylase	-
BV085_RS05410	1078715..1080250	-	1536	WP_005686206.1	L-2,4-diaminobutyrate decarboxylase	-
BV085_RS05415	1080270..1080668	-	399	WP_005651560.1	tRNA(fMet)-specific endonuclease VapC	Toxin
BV085_RS05420	1080668..1080901	-	234	WP_005686209.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
BV085_RS05425	1081048..1082412	-	1365	WP_005686211.1	diaminobutyrate--2-oxoglutarate transaminase	-
BV085_RS05430	1082759..1082929	-	171	WP_005613503.1	50S ribosomal protein L33	-
BV085_RS05435	1082941..1083177	-	237	WP_005542826.1	50S ribosomal protein L28	-
BV085_RS05440	1083391..1084056	-	666	WP_105210541.1	DNA repair protein RadC	-
BV085_RS05445	1084220..1085422	+	1203	WP_005686217.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15115.46 Da
• Isoelectric Point: 8.0514

>T73701 WP_005651560.1 NZ_CP020007:c1080668-1080270 [Haemophilus influenzae]
VLKYMLDTNIVIYVIKRRPLEILSRFNQNAGKMCVSSITVAELYYGAEKSEYPERNIAVIEDFLSRLTILDYQPKHAAHF
GNIKAELSKQGKLIGENDIHIAAHARSEGLILVSNNLREFERVIALRTENWV

Nucleotide

• Length: 399 bp

>T73701 NZ_CP020007:c1080668-1080270 [Haemophilus influenzae]
GTGTTGAAATATATGCTTGATACTAACATTGTGATTTACGTGATTAAGCGTAGGCCATTGGAAATTCTTTCTCGCTTTAA
TCAAAATGCAGGAAAAATGTGCGTGAGTAGCATTACTGTGGCTGAACTCTATTATGGAGCTGAAAAAAGCGAATATCCCG
AAAGAAACATTGCGGTTATTGAAGATTTCTTATCACGCCTCACCATTTTAGATTACCAACCAAAGCATGCAGCTCATTTC
GGCAATATTAAAGCTGAACTCTCTAAACAAGGGAAATTGATTGGCGAAAATGATATTCATATAGCGGCTCATGCTCGTAG
TGAAGGGTTAATTCTGGTTAGTAACAATTTAAGAGAATTTGAGCGAGTTATAGCATTACGTACAGAAAATTGGGTGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8911.01 Da
• Isoelectric Point: 4.4013

>AT73701 WP_005686209.1 NZ_CP020007:c1080901-1080668 [Haemophilus influenzae]
MIETSVFMTNRSQAVRLPAEVRFSEEIKKLSVRVSGSDRILSPLNQSWDSFFLNDQAVSDDFMNEREIAFQPEREAL

Nucleotide

• Length: 234 bp

>AT73701 NZ_CP020007:c1080901-1080668 [Haemophilus influenzae]
ATGATTGAAACCTCTGTTTTTATGACTAATCGAAGTCAAGCTGTGCGTTTGCCCGCTGAAGTTCGCTTTTCTGAAGAAAT
CAAAAAATTATCGGTTCGTGTATCGGGCAGTGATCGGATTTTATCGCCTCTCAACCAATCTTGGGATAGTTTTTTCTTGA
ATGATCAAGCAGTTAGCGATGATTTTATGAATGAACGAGAAATTGCATTTCAACCAGAGCGTGAGGCTTTATAG

Structures

Toxin

• AlphaFold DB: Q4QLW0