Detailed information of TA system TA073685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2567385..2568363
• Replicon: chromosome
• Accession: NZ_CP020002
• Organism: Bacillus thuringiensis strain Bacillus thuringiensis L-7601

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: B4918_RS13570
• Protein ID: WP_044784359.1
• Coordinates: 2567385..2568122 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: B7IX39
• Locus tag: B4918_RS13575
• Protein ID: WP_000237817.1
• Coordinates: 2568235..2568363 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4918_RS13535	2562976..2563383	+	408	WP_000072495.1	VOC family protein	-
B4918_RS13540	2563728..2564510	+	783	Protein_2518	class I SAM-dependent methyltransferase	-
B4918_RS13545	2564664..2565509	-	846	Protein_2519	alpha-keto acid decarboxylase family protein	-
B4918_RS13550	2565485..2565653	-	169	Protein_2520	hypothetical protein	-
B4918_RS13555	2565761..2566243	+	483	WP_000191905.1	MarR family winged helix-turn-helix transcriptional regulator	-
B4918_RS13560	2566409..2567146	+	738	WP_079245515.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
B4918_RS13570	2567385..2568122	+	738	WP_044784359.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
B4918_RS13575	2568235..2568363	+	129	WP_000237817.1	hypothetical protein	Antitoxin
B4918_RS13580	2568436..2568612	+	177	WP_000852629.1	stage II sporulation protein SB	-
B4918_RS13585	2568631..2569020	-	390	WP_000712655.1	YxeA family protein	-
B4918_RS13590	2569232..2570701	+	1470	WP_000287541.1	beta-Ala-His dipeptidase	-
B4918_RS13595	2571014..2571823	+	810	WP_000678376.1	C39 family peptidase	-
B4918_RS13600	2571849..2572454	+	606	WP_000527414.1	hypothetical protein	-
B4918_RS13605	2572595..2572846	-	252	WP_000821942.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28369.10 Da
• Isoelectric Point: 8.2998

>T73685 WP_044784359.1 NZ_CP020002:2567385-2568122 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFLDSYGEKFGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T73685 NZ_CP020002:2567385-2568122 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACCACAATTATTCAAAATGTTAATCCAGCAGTTTTCGGTACGATGG
AATGGCATACGGAAGAAGAATATACAAAGAGCTTAAATGTATTTTTAGATTCATATGGGGAAAAATTTGGCGCGAAAATT
GTTGTTTTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCAGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGATG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4981.37 Da
• Isoelectric Point: 3.9150

>AT73685 WP_000237817.1 NZ_CP020002:2568235-2568363 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDALQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT73685 NZ_CP020002:2568235-2568363 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATGC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: B7IX39