Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 239445..240087 Replicon chromosome
Accession NZ_CP020002
Organism Bacillus thuringiensis strain Bacillus thuringiensis L-7601

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag B4918_RS01405 Protein ID WP_000635965.1
Coordinates 239737..240087 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag B4918_RS01400 Protein ID WP_000004570.1
Coordinates 239445..239732 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4918_RS01375 234760..235722 + 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -
B4918_RS01380 235715..236287 - 573 WP_000908529.1 rhomboid family intramembrane serine protease -
B4918_RS01385 236381..236740 + 360 WP_000583418.1 holo-ACP synthase -
B4918_RS01390 236897..237847 + 951 WP_002084009.1 outer membrane lipoprotein carrier protein LolA -
B4918_RS01395 237965..239134 + 1170 WP_000390595.1 alanine racemase -
B4918_RS01400 239445..239732 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
B4918_RS01405 239737..240087 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
B4918_RS01410 240155..242323 + 2169 WP_000426209.1 RNA-binding transcriptional accessory protein -
B4918_RS01415 242381..242497 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
B4918_RS01420 242693..243151 + 459 WP_000343552.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T73684 WP_000635965.1 NZ_CP020002:239737-240087 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T73684 NZ_CP020002:239737-240087 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT73684 WP_000004570.1 NZ_CP020002:239445-239732 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT73684 NZ_CP020002:239445-239732 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References