Detailed information of TA system TA073676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 1414960..1415511
• Replicon: chromosome
• Accession: NZ_CP019998
• Organism: Arthrospira platensis C1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B5VX51
• Locus tag: APLC1_RS07005
• Protein ID: WP_006623765.1
• Coordinates: 1414960..1415220 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: APLC1_RS07010
• Protein ID: WP_006623767.1
• Coordinates: 1415242..1415511 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APLC1_RS06970 (APLC1_1489)	1410508..1411120	+	613	Protein_1385	IS607 family transposase	-
APLC1_RS06975	1411083..1411412	+	330	WP_245394360.1	hypothetical protein	-
APLC1_RS06980 (APLC1_1490)	1411419..1411943	+	525	WP_260378740.1	hypothetical protein	-
APLC1_RS06985 (APLC1_1491)	1412004..1412879	+	876	WP_108614679.1	IS630-like element ISAtsp1 family transposase	-
APLC1_RS06990	1412897..1413151	+	255	Protein_1389	transposase	-
APLC1_RS06995	1413365..1413961	-	597	Protein_1390	IS630 family transposase	-
APLC1_RS07000	1414032..1414909	+	878	Protein_1391	IS630 family transposase	-
APLC1_RS07005 (APLC1_1496)	1414960..1415220	+	261	WP_006623765.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
APLC1_RS07010	1415242..1415511	+	270	WP_006623767.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
APLC1_RS07015 (APLC1_1497)	1415681..1417222	+	1542	WP_006623768.1	type I restriction-modification system subunit M	-
APLC1_RS07020 (APLC1_1498)	1417219..1418273	+	1055	Protein_1395	PDDEXK nuclease domain-containing protein	-
APLC1_RS07025 (APLC1_1500)	1418270..1418839	+	570	WP_231296326.1	restriction endonuclease subunit S	-
APLC1_RS07030	1418968..1419081	+	114	WP_081587642.1	type II toxin-antitoxin system RelE/ParE family toxin	-
APLC1_RS07035 (APLC1_1501)	1419091..1419399	+	309	WP_006623772.1	HigA family addiction module antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (10-49)

Antitoxin

• mRNA interferase PemK-like (2-65)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9965.27 Da
• Isoelectric Point: 4.6412

>T73676 WP_006623765.1 NZ_CP019998:1414960-1415220 [Arthrospira platensis C1]
MSKMIRTRIVKIGNSQGVRIPKLLLEQSGIQTDVEIEVEGDHLTIRNARRLRTGWEQAFAAMAKEQDDVLLDPVNSTDWE
QSEWEW

Nucleotide

• Length: 261 bp

>T73676 NZ_CP019998:1414960-1415220 [Arthrospira platensis C1]
ATGAGCAAAATGATTAGAACCCGAATCGTTAAAATTGGCAACTCCCAAGGTGTGCGTATTCCTAAACTGCTTTTAGAGCA
AAGTGGTATTCAGACAGACGTTGAAATCGAAGTGGAGGGCGATCATCTCACGATTCGCAACGCACGACGGTTACGCACAG
GCTGGGAGCAAGCCTTTGCAGCAATGGCGAAAGAACAAGATGATGTTCTTCTCGATCCAGTCAACAGTACCGATTGGGAG
CAAAGCGAATGGGAGTGGTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9862.49 Da
• Isoelectric Point: 8.2619

>AT73676 WP_006623767.1 NZ_CP019998:1415242-1415511 [Arthrospira platensis C1]
MVNLDPTIGSEIQKKRPCVIISPDEMNGYITTVIIAPMTTKGKAYPTRISCQFQGKEGQIVLDQTPSIKLDWSKRCFPNE
PRDGAIALP

Nucleotide

• Length: 270 bp

>AT73676 NZ_CP019998:1415242-1415511 [Arthrospira platensis C1]
TTGGTTAATCTTGACCCCACAATTGGCAGTGAAATTCAGAAAAAACGACCCTGTGTGATTATCTCACCGGATGAAATGAA
CGGTTATATTACCACGGTGATTATTGCCCCAATGACGACAAAAGGGAAAGCCTACCCGACGCGAATTTCCTGCCAGTTTC
AGGGTAAGGAAGGGCAAATTGTTCTCGACCAAACACCATCGATAAAGCTCGATTGGTCAAAAAGATGTTTTCCCAATGAG
CCAAGGGACGGCGCGATCGCTCTACCCTGA

Structures

Toxin

• AlphaFold DB: A0A7G7W1U1