Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 33577..34714 Replicon plasmid p268-3
Accession NZ_CP019995
Organism Enterococcus faecium isolate 2014-VREF-268

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag B4W81_RS17445 Protein ID WP_001284311.1
Coordinates 33577..34440 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag B4W81_RS17450 Protein ID WP_000301765.1
Coordinates 34442..34714 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4W81_RS17415 29149..30444 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
B4W81_RS17420 30689..30940 + 252 Protein_39 GNAT family N-acetyltransferase -
B4W81_RS17425 31033..31827 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
B4W81_RS17430 32237..32320 + 84 Protein_41 peptide-binding protein -
B4W81_RS17435 32369..32452 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
B4W81_RS17440 32577..33314 + 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
B4W81_RS17445 33577..34440 - 864 WP_001284311.1 toxin zeta Toxin
B4W81_RS17450 34442..34714 - 273 WP_000301765.1 antitoxin Antitoxin
B4W81_RS17455 34731..34940 - 210 WP_000527318.1 peptide-binding protein -
B4W81_RS17460 35038..35295 - 258 WP_002347171.1 hypothetical protein -
B4W81_RS17465 35351..36031 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
B4W81_RS17470 35936..36803 + 868 Protein_49 replication initiation factor domain-containing protein -
B4W81_RS17475 36800..37033 + 234 WP_002303408.1 hypothetical protein -
B4W81_RS17480 37026..37445 + 420 WP_044384217.1 hypothetical protein -
B4W81_RS17485 37424..37762 + 339 WP_171970646.1 hypothetical protein -
B4W81_RS17490 37766..38128 + 363 WP_002298418.1 hypothetical protein -
B4W81_RS17980 38248..38438 + 191 Protein_54 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid VanHAX / aph(3')-III / erm(B) - 1..58211 58211
- inside IScluster/Tn VanHAX / aph(3')-III / erm(B) - 17821..55917 38096


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T73672 WP_001284311.1 NZ_CP019995:c34440-33577 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T73672 NZ_CP019995:c34440-33577 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT73672 WP_000301765.1 NZ_CP019995:c34714-34442 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT73672 NZ_CP019995:c34714-34442 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References