Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 239659..240178 Replicon plasmid p268-1
Accession NZ_CP019993
Organism Enterococcus faecium isolate 2014-VREF-268

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag B4W81_RS16595 Protein ID WP_000588503.1
Coordinates 239659..239916 (-) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag B4W81_RS16600 Protein ID WP_000388479.1
Coordinates 239909..240178 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4W81_RS16565 234881..235561 + 681 WP_010861579.1 IS6-like element IS1216 family transposase -
B4W81_RS16570 235750..236334 - 585 WP_002299575.1 recombinase family protein -
B4W81_RS16575 236545..236748 - 204 WP_002299573.1 hypothetical protein -
B4W81_RS16580 236758..237180 - 423 WP_002303116.1 hypothetical protein -
B4W81_RS16585 237418..238032 + 615 WP_001261742.1 recombinase family protein -
B4W81_RS16590 238197..239021 - 825 WP_079200806.1 hypothetical protein -
B4W81_RS17965 239045..239200 - 156 WP_002347142.1 hypothetical protein -
B4W81_RS16595 239659..239916 - 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
B4W81_RS16600 239909..240178 - 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
B4W81_RS16605 240426..241574 - 1149 WP_002287525.1 IS200/IS605 family element transposase accessory protein TnpB -
B4W81_RS16610 241591..241995 - 405 WP_002287522.1 IS200/IS605-like element ISEfa4 family transposase -
B4W81_RS16615 242200..242379 - 180 WP_001120991.1 hypothetical protein -
B4W81_RS16625 242760..243467 - 708 WP_002303117.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(2'')-Ia / aac(6')-aph(2'') - 1..243818 243818
- inside IScluster/Tn - - 234881..241995 7114


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T73671 WP_000588503.1 NZ_CP019993:c239916-239659 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T73671 NZ_CP019993:c239916-239659 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT73671 WP_000388479.1 NZ_CP019993:c240178-239909 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT73671 NZ_CP019993:c240178-239909 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References