Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | Txe-Axe/Txe-RelB |
Location | 239659..240178 | Replicon | plasmid p268-1 |
Accession | NZ_CP019993 | ||
Organism | Enterococcus faecium isolate 2014-VREF-268 |
Toxin (Protein)
Gene name | Txe | Uniprot ID | Q848U6 |
Locus tag | B4W81_RS16595 | Protein ID | WP_000588503.1 |
Coordinates | 239659..239916 (-) | Length | 86 a.a. |
Antitoxin (Protein)
Gene name | Axe | Uniprot ID | Q83WJ8 |
Locus tag | B4W81_RS16600 | Protein ID | WP_000388479.1 |
Coordinates | 239909..240178 (-) | Length | 90 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
B4W81_RS16565 | 234881..235561 | + | 681 | WP_010861579.1 | IS6-like element IS1216 family transposase | - |
B4W81_RS16570 | 235750..236334 | - | 585 | WP_002299575.1 | recombinase family protein | - |
B4W81_RS16575 | 236545..236748 | - | 204 | WP_002299573.1 | hypothetical protein | - |
B4W81_RS16580 | 236758..237180 | - | 423 | WP_002303116.1 | hypothetical protein | - |
B4W81_RS16585 | 237418..238032 | + | 615 | WP_001261742.1 | recombinase family protein | - |
B4W81_RS16590 | 238197..239021 | - | 825 | WP_079200806.1 | hypothetical protein | - |
B4W81_RS17965 | 239045..239200 | - | 156 | WP_002347142.1 | hypothetical protein | - |
B4W81_RS16595 | 239659..239916 | - | 258 | WP_000588503.1 | Txe/YoeB family addiction module toxin | Toxin |
B4W81_RS16600 | 239909..240178 | - | 270 | WP_000388479.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
B4W81_RS16605 | 240426..241574 | - | 1149 | WP_002287525.1 | IS200/IS605 family element transposase accessory protein TnpB | - |
B4W81_RS16610 | 241591..241995 | - | 405 | WP_002287522.1 | IS200/IS605-like element ISEfa4 family transposase | - |
B4W81_RS16615 | 242200..242379 | - | 180 | WP_001120991.1 | hypothetical protein | - |
B4W81_RS16625 | 242760..243467 | - | 708 | WP_002303117.1 | hypothetical protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | aph(2'')-Ia / aac(6')-aph(2'') | - | 1..243818 | 243818 | |
- | inside | IScluster/Tn | - | - | 234881..241995 | 7114 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 86 a.a. Molecular weight: 10206.53 Da Isoelectric Point: 8.4369
>T73671 WP_000588503.1 NZ_CP019993:c239916-239659 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY
Download Length: 258 bp
>T73671 NZ_CP019993:c239916-239659 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA
Antitoxin
Download Length: 90 a.a. Molecular weight: 10373.51 Da Isoelectric Point: 4.4346
>AT73671 WP_000388479.1 NZ_CP019993:c240178-239909 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD
Download Length: 270 bp
>AT73671 NZ_CP019993:c240178-239909 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A828ZXQ8 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A828ZWU7 |