Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 654162..654691 Replicon chromosome
Accession NZ_CP019992
Organism Enterococcus faecium isolate 2014-VREF-268

Toxin (Protein)


Gene name relE Uniprot ID Q3XX67
Locus tag B4W81_RS03360 Protein ID WP_002296560.1
Coordinates 654162..654428 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A829F3T0
Locus tag B4W81_RS03365 Protein ID WP_002296559.1
Coordinates 654428..654691 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4W81_RS03335 649530..650705 + 1176 WP_002294192.1 betaine/proline/choline family ABC transporter ATP-binding protein -
B4W81_RS03340 650709..651350 + 642 WP_002293405.1 ABC transporter permease -
B4W81_RS03345 651347..652264 + 918 WP_002302664.1 osmoprotectant ABC transporter substrate-binding protein -
B4W81_RS03350 652261..652932 + 672 WP_002302663.1 ABC transporter permease -
B4W81_RS03355 653473..653961 - 489 WP_002296561.1 mannosyl-glycoprotein endo-beta-N-acetylglucosamidase -
B4W81_RS03360 654162..654428 - 267 WP_002296560.1 Txe/YoeB family addiction module toxin Toxin
B4W81_RS03365 654428..654691 - 264 WP_002296559.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
B4W81_RS03375 655167..655352 + 186 WP_002296558.1 transcriptional regulator -
B4W81_RS03380 655494..657317 - 1824 WP_002294202.1 APC family permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10660.13 Da        Isoelectric Point: 9.9931

>T73669 WP_002296560.1 NZ_CP019992:c654428-654162 [Enterococcus faecium]
MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQSFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEI
YSAWTHYE

Download         Length: 267 bp

>T73669 NZ_CP019992:c654428-654162 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10046.05 Da        Isoelectric Point: 4.1207

>AT73669 WP_002296559.1 NZ_CP019992:c654691-654428 [Enterococcus faecium]
MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIGLEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNID
DIDWEAL

Download         Length: 264 bp

>AT73669 NZ_CP019992:c654691-654428 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FCW8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3T0

References