Detailed information of TA system TA073667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 232278..232797
• Replicon: plasmid p63-1
• Accession: NZ_CP019989
• Organism: Enterococcus faecium isolate 2014-VREF-63

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: B4W80_RS16065
• Protein ID: WP_000588503.1
• Coordinates: 232278..232535 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: B4W80_RS16070
• Protein ID: WP_000388479.1
• Coordinates: 232528..232797 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4W80_RS16035	227500..228180	+	681	WP_010861579.1	IS6-like element IS1216 family transposase	-
B4W80_RS16040	228369..228953	-	585	WP_002299575.1	recombinase family protein	-
B4W80_RS16045	229164..229367	-	204	WP_002299573.1	hypothetical protein	-
B4W80_RS16050	229377..229799	-	423	WP_002303116.1	hypothetical protein	-
B4W80_RS16055	230037..230651	+	615	WP_001261742.1	recombinase family protein	-
B4W80_RS16060	230816..231640	-	825	WP_001809248.1	hypothetical protein	-
B4W80_RS17010	231664..231819	-	156	WP_002347142.1	hypothetical protein	-
B4W80_RS16065	232278..232535	-	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
B4W80_RS16070	232528..232797	-	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
B4W80_RS16075	232928..233107	-	180	WP_001120991.1	hypothetical protein	-
B4W80_RS16085	233488..234195	-	708	WP_002303117.1	hypothetical protein	-
B4W80_RS16090	234188..234625	-	438	WP_002300851.1	hypothetical protein	-
B4W80_RS16095	234640..234891	-	252	WP_002296238.1	hypothetical protein	-
B4W80_RS16100	234891..235097	-	207	WP_002347218.1	hypothetical protein	-
B4W80_RS16110	235534..235794	-	261	WP_002295623.1	hypothetical protein	-
B4W80_RS16115	235869..236072	+	204	WP_002316074.1	putative holin-like toxin	-
B4W80_RS16120	236213..236698	-	486	WP_002303313.1	single-stranded DNA-binding protein	-
B4W80_RS16125	236701..237600	-	900	WP_002303312.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	VanHAX / aac(6')-aph(2'')	-	1..287502	287502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T73667 WP_000588503.1 NZ_CP019989:c232535-232278 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T73667 NZ_CP019989:c232535-232278 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT73667 WP_000388479.1 NZ_CP019989:c232797-232528 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT73667 NZ_CP019989:c232797-232528 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7