Detailed information of TA system TA073665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 101673..102223
• Replicon: plasmid unnamed
• Accession: NZ_CP019987
• Organism: Citrobacter werkmanii strain BF-6

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: B2G73_RS24880
• Protein ID: WP_007372286.1
• Coordinates: 101673..101981 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: B2G73_RS24885
• Protein ID: WP_007372285.1
• Coordinates: 101984..102223 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2G73_RS24840	96755..97198	-	444	WP_007372295.1	hypothetical protein	-
B2G73_RS24845	97436..97774	+	339	WP_007372294.1	hypothetical protein	-
B2G73_RS24850	98137..98463	-	327	WP_007372293.1	hypothetical protein	-
B2G73_RS24855	98547..98945	-	399	WP_007372292.1	hypothetical protein	-
B2G73_RS24860	98956..99198	-	243	WP_007372291.1	hypothetical protein	-
B2G73_RS24865	99545..100015	-	471	WP_007372290.1	hypothetical protein	-
B2G73_RS24870	100098..100715	-	618	WP_007372289.1	hypothetical protein	-
B2G73_RS24875	100785..101339	-	555	WP_007372288.1	hypothetical protein	-
B2G73_RS25890	101494..101652	-	159	WP_016241558.1	hypothetical protein	-
B2G73_RS24880	101673..101981	-	309	WP_007372286.1	CcdB family protein	Toxin
B2G73_RS24885	101984..102223	-	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
B2G73_RS25945	102362..102826	-	465	WP_008786596.1	hypothetical protein	-
B2G73_RS24895	102823..103494	-	672	WP_007372283.1	hypothetical protein	-
B2G73_RS24900	103955..104587	-	633	WP_007372282.1	hypothetical protein	-
B2G73_RS25895	104603..104743	-	141	WP_007372281.1	hypothetical protein	-
B2G73_RS24905	104891..105349	-	459	WP_008786599.1	hypothetical protein	-
B2G73_RS24910	105402..105599	-	198	WP_032155220.1	Lar family restriction alleviation protein	-
B2G73_RS24915	105622..106317	-	696	WP_007372278.1	hypothetical protein	-
B2G73_RS24920	106448..106639	-	192	WP_016241560.1	hypothetical protein	-
B2G73_RS24925	106821..107216	-	396	WP_024196081.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..212549	212549

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T73665 WP_007372286.1 NZ_CP019987:c101981-101673 [Citrobacter werkmanii]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T73665 NZ_CP019987:c101981-101673 [Citrobacter werkmanii]
ATGCAATATACCGTTTACCGCAATCCCGGCAACAGCCAGGCATACCCTTACCTGCTGGATATACAGAGCGACATCATTGG
CGAGTTAAATACCCGCCTGGTGATCCCCCTTCATCGGCTTAAAAAAGGAGCCTCGGCCCCGGTTGCGCGATTAACTCCGG
TCATACAGGTGGAAGGGAATGACGTTATCCTGATGACGCATGAAATGGCTTCAGTCCGCGTGAAGCAGCTCGGTCAGGCG
GTCATGGACGCATCTCCGTTCCGGCACACTATCAAAAGTGCGGTGGACTTCCTGCTGGATGGTTTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT73665 WP_007372285.1 NZ_CP019987:c102223-101984 [Citrobacter werkmanii]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT73665 NZ_CP019987:c102223-101984 [Citrobacter werkmanii]
ATGCAGGCTACAGCTGCTCGCCAGAAAAAAACCGTCAGTGTCACCCTTGAGCCAATGCTCGTACAGCAGGCAAAAGACGC
TGGGATTAACTTGTCCGCCACGCTGGCAGAAGCGCTTCAGAGCAAACTGAAGGCCAGTGCAGCGGAAGAATGGAAACGAC
AGAATCGTGCTGGTATTCAGGAGTTGAACCGCATCACTGAAGAGCATGGGTTACTGTCTGACGAATACAGGACGTTCTGA

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3