Detailed information of TA system TA073664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 94023..94924
• Replicon: plasmid unnamed
• Accession: NZ_CP019987
• Organism: Citrobacter werkmanii strain BF-6

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A837LJP8
• Locus tag: B2G73_RS24815
• Protein ID: WP_007372300.1
• Coordinates: 94466..94924 (+)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: B2G73_RS24810
• Protein ID: WP_008786588.1
• Coordinates: 94023..94469 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2G73_RS24785	90059..90901	-	843	WP_007372307.1	HNH endonuclease	-
B2G73_RS25885	91469..91633	-	165	WP_007372306.1	hypothetical protein	-
B2G73_RS24790	91920..92363	-	444	WP_007372305.1	hypothetical protein	-
B2G73_RS24795	92423..92701	-	279	WP_007372304.1	hypothetical protein	-
B2G73_RS24800	92673..93515	-	843	WP_007372303.1	hypothetical protein	-
B2G73_RS24805	93557..93874	-	318	WP_007372302.1	hypothetical protein	-
B2G73_RS24810	94023..94469	+	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
B2G73_RS24815	94466..94924	+	459	WP_007372300.1	RES family NAD+ phosphorylase	Toxin
B2G73_RS24820	94968..95198	+	231	WP_007372299.1	hypothetical protein	-
B2G73_RS24825	95266..95532	-	267	WP_007372298.1	hypothetical protein	-
B2G73_RS24830	95582..96136	-	555	WP_007372297.1	hypothetical protein	-
B2G73_RS24835	96295..96636	-	342	WP_007372296.1	hypothetical protein	-
B2G73_RS24840	96755..97198	-	444	WP_007372295.1	hypothetical protein	-
B2G73_RS24845	97436..97774	+	339	WP_007372294.1	hypothetical protein	-
B2G73_RS24850	98137..98463	-	327	WP_007372293.1	hypothetical protein	-
B2G73_RS24855	98547..98945	-	399	WP_007372292.1	hypothetical protein	-
B2G73_RS24860	98956..99198	-	243	WP_007372291.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..212549	212549

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 16975.40 Da
• Isoelectric Point: 4.5730

>T73664 WP_007372300.1 NZ_CP019987:94466-94924 [Citrobacter werkmanii]
VILYRLTKTRYLMTAWSGLGAKEAGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T73664 NZ_CP019987:94466-94924 [Citrobacter werkmanii]
GTGATCCTTTACCGACTGACCAAGACCCGTTATCTGATGACTGCGTGGTCTGGTCTGGGTGCGAAAGAAGCGGGGGGCCG
CTGGAACAGCGTAGGCACTGCAATGGTCTACCTGTCTGAAACGGCCAGCCTTACCATGCTTGAAACGCTGGTACACATTC
ACGCGCCGCAGTTACTGGACGACTTTACACTGCTGAGTCTTGATGTACCGGATGATCAGATCCAGACCTTCGACATGAGC
CGTCTCCCTGATAACTGGGCGAGTGAGGATGCTCCTGCTGAGCTGGCGCTCTACGGTGATAACTGGGCTGAAAGCGGTTC
TTCTATCGCGCTGCGTATTCCAAGTGCGTTGTCTCCGGTTGAATTTAACTACTTGCTGAATCCGGCACACCCTGAACTCT
TTGACCTGATGGCTACGGTTAAGAAAATTCCGTTCCGGTTTGATTCCCGCCTGAAATAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT73664 WP_008786588.1 NZ_CP019987:94023-94469 [Citrobacter werkmanii]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT73664 NZ_CP019987:94023-94469 [Citrobacter werkmanii]
ATGACGATGAAAGTTTTTTCTCCAGACGTTACCAAAACGAATCAGCACTGCTTATGGCAGGTTGCTGGGCTGGATAATGA
CGGTATCGCGCTGATGGATAAAATCAATCACGGTCTCGATGGCACAGTAGCTAAGCACATTTCTGAATGGGCTAACATCA
CGCCGTCTGAGCTGCGCAAAATGTCGGGTATACCTAATACGACGTTTAATCGTAGCATCAAAGACCGTTTCACTGCCGAT
CAGAGCGAACGTCTGGTGCGCATTATCCGCGTAATTGAACGCGCTGTAGAGCTGTTTGAAGGTGACAAAGAAGCGGCGCA
CAAATGGCTGAACGAAGCGAACCGTGGTCTGAGCTGGAAATCACCGGCAGAGCTTGTTTCTTCTGAGACAGGTGCGCTTG
AAGTGATGCGCCTGATTACGCGTATTGAGCACGGGGTATACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A837LJP8