Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3577636..3578252 Replicon chromosome
Accession NZ_CP019986
Organism Citrobacter werkmanii strain BF-6

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag B2G73_RS17560 Protein ID WP_061382591.1
Coordinates 3577636..3578010 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A8E9YHE9
Locus tag B2G73_RS17565 Protein ID WP_001523745.1
Coordinates 3578010..3578252 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2G73_RS17545 3575139..3576041 + 903 WP_042313505.1 formate dehydrogenase O subunit beta -
B2G73_RS17550 3576038..3576673 + 636 WP_042313508.1 formate dehydrogenase cytochrome b556 subunit -
B2G73_RS17555 3576670..3577599 + 930 WP_042313511.1 formate dehydrogenase accessory protein FdhE -
B2G73_RS17560 3577636..3578010 - 375 WP_061382591.1 type II toxin-antitoxin system VapC family toxin Toxin
B2G73_RS17565 3578010..3578252 - 243 WP_001523745.1 ribbon-helix-helix domain-containing protein Antitoxin
B2G73_RS17570 3578457..3579365 + 909 WP_079225728.1 alpha/beta hydrolase -
B2G73_RS17575 3579430..3579672 + 243 WP_069324888.1 type II toxin-antitoxin system ParD family antitoxin -
B2G73_RS17580 3579665..3579952 + 288 WP_079225729.1 type II toxin-antitoxin system RelE/ParE family toxin -
B2G73_RS17585 3579958..3580899 - 942 WP_032940213.1 fatty acid biosynthesis protein FabY -
B2G73_RS17590 3580944..3581381 - 438 WP_079225730.1 D-tyrosyl-tRNA(Tyr) deacylase -
B2G73_RS17595 3581378..3582250 - 873 WP_042313529.1 virulence factor BrkB family protein -
B2G73_RS17600 3582244..3582843 - 600 WP_038635879.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3583179..3583397 218


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13713.92 Da        Isoelectric Point: 8.5343

>T73662 WP_061382591.1 NZ_CP019986:c3578010-3577636 [Citrobacter werkmanii]
MVKGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRIALGAFNIIGVSQDIAERSVNI
RQEYGMKLPDAIILATAQIHHLTLVTRNTKDFASISGVVTPYTL

Download         Length: 375 bp

>T73662 NZ_CP019986:c3578010-3577636 [Citrobacter werkmanii]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGGCGAAGTGAAGCAAAACTGGCGAT
AGAAACATGGCCGCCACAGAACGCAATCAGTCTCATTACCTGGATGGAGGTGATGGTAGGCGCAAAAAAATACCATCAGG
AGGCCCGTACTCGCATTGCGCTAGGCGCTTTTAACATTATTGGAGTTTCACAGGATATTGCTGAGCGAAGCGTGAATATC
AGGCAAGAATACGGGATGAAATTGCCGGATGCCATTATCCTGGCTACTGCGCAGATTCATCATCTGACACTGGTTACGCG
TAATACCAAAGATTTTGCAAGCATATCCGGTGTGGTCACACCGTATACGTTATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9437.77 Da        Isoelectric Point: 4.4463

>AT73662 WP_001523745.1 NZ_CP019986:c3578252-3578010 [Citrobacter werkmanii]
MMAGMDMGRILLDLSDDVIKRLDDLKVQRNLPRAELLREAVEQYLERQDRAETTISKALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT73662 NZ_CP019986:c3578252-3578010 [Citrobacter werkmanii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGACGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAAACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References