Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2470735..2471355 Replicon chromosome
Accession NZ_CP019986
Organism Citrobacter werkmanii strain BF-6

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag B2G73_RS12285 Protein ID WP_002892050.1
Coordinates 2471137..2471355 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag B2G73_RS12280 Protein ID WP_003021733.1
Coordinates 2470735..2471109 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2G73_RS12270 2465882..2467075 + 1194 WP_079225192.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
B2G73_RS12275 2467098..2470247 + 3150 WP_038638345.1 multidrug efflux RND transporter permease subunit -
B2G73_RS12280 2470735..2471109 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
B2G73_RS12285 2471137..2471355 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
B2G73_RS12290 2471537..2472088 + 552 WP_042310052.1 maltose O-acetyltransferase -
B2G73_RS12295 2472205..2472675 + 471 WP_042310054.1 YlaC family protein -
B2G73_RS12300 2472749..2472889 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
B2G73_RS12305 2472891..2473151 - 261 WP_038638331.1 type B 50S ribosomal protein L31 -
B2G73_RS12310 2473339..2474892 + 1554 WP_042310056.1 EAL domain-containing protein -
B2G73_RS12315 2474944..2475297 - 354 WP_042310059.1 DUF1428 family protein -
B2G73_RS12320 2475362..2475991 - 630 WP_079225194.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T73661 WP_002892050.1 NZ_CP019986:2471137-2471355 [Citrobacter werkmanii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T73661 NZ_CP019986:2471137-2471355 [Citrobacter werkmanii]
ATGTCTGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGCTGCCAGACAATTGACACGCTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCGGATCACCGCCTTGCTGAAT
TGACCATGAATAAGCTCTACGACAAGATTCCAACTTCCGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT73661 WP_003021733.1 NZ_CP019986:2470735-2471109 [Citrobacter werkmanii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT73661 NZ_CP019986:2470735-2471109 [Citrobacter werkmanii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAACGACCCTACCTCTGCAACCAGCTTACAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAATCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References