Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 472140..472695 Replicon chromosome
Accession NZ_CP019985
Organism Alkalihalobacillus clausii strain DSM 8716

Toxin (Protein)


Gene name pemK Uniprot ID A0A268RU48
Locus tag BC8716_RS02315 Protein ID WP_062751375.1
Coordinates 472140..472490 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A268RU80
Locus tag BC8716_RS02320 Protein ID WP_050748939.1
Coordinates 472495..472695 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BC8716_RS02280 467584..468369 - 786 WP_011245671.1 RNA polymerase sigma factor SigB -
BC8716_RS02285 468329..468811 - 483 WP_094423745.1 anti-sigma B factor RsbW -
BC8716_RS02290 468811..469140 - 330 WP_094423746.1 STAS domain-containing protein -
BC8716_RS02295 469205..470221 - 1017 WP_094423747.1 PP2C family protein-serine/threonine phosphatase -
BC8716_RS02300 470222..470653 - 432 WP_094423748.1 anti-sigma regulatory factor -
BC8716_RS02305 470656..471012 - 357 WP_094423749.1 STAS domain-containing protein -
BC8716_RS02310 471030..471848 - 819 WP_094423750.1 RsbT co-antagonist protein RsbRA -
BC8716_RS02315 472140..472490 - 351 WP_062751375.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BC8716_RS02320 472495..472695 - 201 WP_050748939.1 hypothetical protein Antitoxin
BC8716_RS02325 472838..474070 - 1233 WP_094423751.1 alanine racemase -
BC8716_RS02330 474219..475241 - 1023 WP_094423752.1 outer membrane lipoprotein carrier protein LolA -
BC8716_RS02335 475361..475714 - 354 WP_094423753.1 holo-ACP synthase -
BC8716_RS02340 475838..476599 + 762 WP_094423754.1 rhomboid family intramembrane serine protease -
BC8716_RS02345 476689..477315 + 627 WP_094423755.1 YitT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12859.96 Da        Isoelectric Point: 9.6233

>T73649 WP_062751375.1 NZ_CP019985:c472490-472140 [Alkalihalobacillus clausii]
VIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNNIGNRFSPTVIVAAITAQIQKAKLPTHVEINASKYGFDRDSVILLEQV
RTIDKQRLTDKITHLDEKMMRSVDKALTISLGLIDF

Download         Length: 351 bp

>T73649 NZ_CP019985:c472490-472140 [Alkalihalobacillus clausii]
GTGATAGTCAAACGCGGCGATGTGTATTTTGCAGATCTCTCCCCCGTTGTCGGCTCAGAACAAGGTGGGGTCAGGCCTGT
ACTCATCATACAAAATAATATCGGTAACCGATTTAGCCCGACAGTGATCGTAGCAGCCATTACAGCCCAAATCCAAAAAG
CAAAACTCCCAACACACGTGGAAATCAATGCGAGCAAATATGGGTTCGACCGGGATTCGGTCATTCTTCTTGAGCAAGTA
CGCACGATTGACAAACAACGACTGACAGATAAAATTACCCATCTTGACGAAAAAATGATGAGGTCTGTTGACAAGGCACT
CACGATCAGTTTGGGATTAATTGATTTTTAA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7509.42 Da        Isoelectric Point: 4.4423

>AT73649 WP_050748939.1 NZ_CP019985:c472695-472495 [Alkalihalobacillus clausii]
VDAVLEQEKWDNEENSNKRAKLKAEIRESMQQGYMEMAKINLTIASEAFLAEEEANHTLTRLVSGV

Download         Length: 201 bp

>AT73649 NZ_CP019985:c472695-472495 [Alkalihalobacillus clausii]
GTGGATGCTGTGCTTGAGCAGGAAAAGTGGGACAACGAAGAAAACAGCAATAAGCGTGCAAAACTAAAAGCTGAAATTCG
TGAGTCTATGCAACAAGGCTATATGGAGATGGCGAAAATCAATCTTACAATTGCATCAGAAGCCTTTCTTGCTGAGGAGG
AAGCGAATCATACGCTGACGCGTCTCGTTAGTGGGGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A268RU48

Antitoxin

Source ID Structure
AlphaFold DB A0A268RU80

References